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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 17, 2013 |
| Title |
Genome-wide analysis of the function of Tlx in regulating mouse brain glioma gene expression |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of the function of Tlx in regulating brain tumor stem cells (BTSCs) at the gene expression level. Based on our knowledge, we know that Tlx is specifically expressed in the neural stem cells in mouse brain. It also functionally regulates neurogenesis during the postnatal stages. The hypothesis tested in the present study is that Tlx influences the maintenance and progression of mouse brain gliomas through regulating the self-renewal of BTSCs and it is also a specific marker of brain tumor stem cells (BTSCs). Results provide important information about the function of Tlx in brain gliomas.
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| Overall design |
Brain gliomas were initiated from the Nestin-CreERT2;Tlx flox/flox;Nestin-Tva transgenic mouse line. After 2.5 weeks, when the brain gliomas were fully developed, tamoxifen was administered through intraperitoneal injection. Tlx was knocked-out from the Cre-expressing littermates, but the littermates without Cre expression maintained intact Tlx function. Then, total RNA was obtained from mouse brain gliomas with severe neurological symptoms from the Tlx knockout and Tlx intact groups.
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| Contributor(s) |
Zhu Z, Khan MA, Gronych J, Bernhardt O, Korshunov A, Radlwimmer B, Lichter P, Liu HK |
| Citation(s) |
24835569 |
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| Submission date |
Apr 16, 2013 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Hai-Kun Liu |
| E-mail(s) |
l.haikun@dkfz.de
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| Phone |
+496221423266
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| Organization name |
Deutsches Krebsforschungszentrum DKFZ
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| Street address |
INF580
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| City |
Heidelberg |
| State/province |
69120 |
| ZIP/Postal code |
D69121 |
| Country |
Germany |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (12)
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| GSM1124007 |
NestinCreERT2;Tlx f/f; N-Tva CRE-, 5619 |
| GSM1124008 |
NestinCreERT3;Tlx f/f; N-Tva CRE-, 5622 |
| GSM1124009 |
NestinCreERT4;Tlx f/f; N-Tva CRE-, 5943 |
| GSM1124010 |
NestinCreERT5;Tlx f/f; N-Tva CRE-, 100312 |
| GSM1124011 |
NestinCreERT6;Tlx f/f; N-Tva CRE-, 13.03_2 |
| GSM1124012 |
NestinCreERT7;Tlx f/f; N-Tva CRE-, 080312 |
| GSM1124013 |
NestinCreERT8;Tlx f/f; N-Tva CRE+, 6105 |
| GSM1124014 |
NestinCreERT9;Tlx f/f; N-Tva CRE+, 5611 |
| GSM1124015 |
NestinCreERT10;Tlx f/f; N-Tva CRE+, 6103 |
| GSM1124016 |
NestinCreERT11;Tlx f/f; N-Tva CRE+, 6102 |
| GSM1124017 |
NestinCreERT12;Tlx f/f; N-Tva CRE+, 5631 |
| GSM1124018 |
NestinCreERT13;Tlx f/f; N-Tva CRE+, 5616 |
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| Relations |
| BioProject |
PRJNA197301 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE46125_RAW.tar |
15.8 Mb |
(http)(custom) |
TAR |
| GSE46125_non-normalized.txt.gz |
5.2 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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