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| Status |
Public on Dec 15, 2013 |
| Title |
Comparative genomic hybridization in traditional fermentative Saccharomyces cerevisiae yeasts |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome variation profiling by array
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| Summary |
We study the genetics, including microarray karyotyping using comparative genomic hybridization, to explore global changes in the genomic DNA of seven S. cerevisiae strains related to traditional fermentations of very different sources comparing to the sequenced S. cerevisiae laboratory strain (S288C). Our final goal is to determine the adaptive evolution of properties of biotechnological interest in Saccharomyces yeasts. Many copy number variations (CNVs) were observed, especially in genes associated to subtelomeric regions and transposon elements. Among the fermentation strains, differential CNV was observed in genes related to sugar transport and metabolism. An outstanding example of diverse CNV is the gen PUT1, involved in proline assimilation, which correlated with the adaptation of the strains to the presence of this nitrogen source in the media.
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| Overall design |
Seven S. cerevisiae strains were obtained from natural environments and different fermentation processes. The S. cerevisiae strain S288C was used as a control for microarray hybridizations. All experiments were performed using duplicate arrays, and Cy5-dCTP and Cy3-dCTP dye-swap assays were performed to reduce dye-specific bias.
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| Contributor(s) |
Ibáñez C, Pérez-Torrado R, Chiva R, Guillamón JM, Barrio E, Querol A |
| Citation(s) |
24334254 |
| |
| Submission date |
Apr 17, 2013 |
| Last update date |
Nov 21, 2014 |
| Contact name |
Clara Ibáñez |
| E-mail(s) |
cibanez@iata.csic.es
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| Organization name |
Institute of Agrochemistry and Food Technology
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| Street address |
Avenue Agustín Escardino, 7
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| City |
Valencia |
| ZIP/Postal code |
46980 |
| Country |
Spain |
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| Platforms (1) |
| GPL13945 |
UHNMAC Yeast 6.4 K array (Y6.4K) version 7 |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA197425 |
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