|
| Status |
Public on Aug 01, 2014 |
| Title |
Identification of miRNAs regulated by TGF-b in human CD8+ T cells (small RNA-seq) |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
The goal of the current study was to identify miRNAs regulated by TGF-b in human CD8+ T cells and analyze the function of these miRNAs in shaping the immunomodulatory effect of TGF-b in these cells. We identified the miR-23a cluster to be upregulated and found that this cluster could target key molecules (IFN-g and LAMP1) involved in immune response by CD8+ T cells.
|
| |
|
| Overall design |
CD8+ T cells were isolated and purified from healthy human peripheral blood of 5 donors and were activated using beads coupled to anti-CD2, anti-CD3 and anti-CD24. They were then treated with 5ng/ml TGF-b, with 1µM SD-208 or left untreated. RNA from these cells were then isolated and used for deep sequencing.
|
| |
|
| Contributor(s) |
Chandran AP, Keller A, Wischhusen J, Eichner N, Meister G |
| Citation(s) |
25030422 |
| |
| Submission date |
Apr 23, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Anoop Chandran P |
| E-mail(s) |
premachand_a@klinik.uni-wuerzburg.de
|
| Organization name |
University of Würzburg
|
| Street address |
Josef-schneiderstrasse
|
| City |
Würzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
| |
|
| Platforms (1) |
|
| Samples (3) |
|
| This SubSeries is part of SuperSeries: |
| GSE46325 |
Identification of miRNAs regulated by TGF-b in human CD8+ T cells |
|
| Relations |
| BioProject |
PRJNA198733 |
| SRA |
SRP021477 |
