|
| Status |
Public on Jul 01, 2014 |
| Title |
Yeast X-Chromosome Associated Protein 5 (Xap5) Functions with H2A.Z to Suppress Aberrant Transcripts [RNA-seq] |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Functional characterization of the Xap5 protein using epistatic genetic interaction analysis (E-MAP), RNA-seq and ChIP-chip along with other growth experiments.
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| |
|
| Overall design |
wild type and mutant cells were grown at 37C in EMM50 and RNA was extracted from exponentially growing cells. Strand specific libraries were constructed with random primers. Ribo zero was used to remove r-RNA. For ChIP-chip Agilent 60mer array was used to analyze DNA recovered by chromatin immunoprecipitation of Xap5 from fission yeast culture.
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| |
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| Contributor(s) |
Anver S, Roguev A, Zofall M, Krogan NJ, Grewal SI, Harmer SL |
| Citation(s) |
24957674 |
| |
| Submission date |
Apr 25, 2013 |
| Last update date |
Nov 16, 2020 |
| Contact name |
Shajahan Anver |
| E-mail(s) |
sanver@ucdavis.edu
|
| Organization name |
University of California Davis
|
| Department |
Plant Biology
|
| Lab |
Harmer Lab
|
| Street address |
1002 LSA, One Shields Av
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13988 |
Illumina HiSeq 2000 (Schizosaccharomyces pombe) |
|
| Samples (13)
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| This SubSeries is part of SuperSeries: |
| GSE46506 |
Yeast X-Chromosome Associated Protein 5 (Xap5) Functions with H2A.Z to Suppress Aberrant Transcripts |
|
| Relations |
| BioProject |
PRJNA198902 |
| SRA |
SRP021515 |
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