|
| Status |
Public on Apr 01, 2007 |
| Title |
Genetic reconstruction of a functional transcriptional regulatory network |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
Although global analyses of transcription factor binding provide
one view of potential transcriptional regulatory networks,
regulation also occurs at levels distinct from transcription
factor binding. Here, we use a genetic approach to identify
targets of transcription factors in yeast and reconstruct a
functional regulatory network. First, we profiled transcriptional
responses in S. cerevisiae strains with individual deletions of
263 transcription factors. Then we used directed-weighted
graph modeling and regulatory epistasis analysis to identify
indirect regulatory relationships between these transcription
factors, and from this we reconstructed a functional
transcriptional regulatory network. The enrichment of
promoter motifs and Gene Ontology annotations provide
insight into the biological functions of the transcription factors.
Data values are X scores and corresponding p-values derived using an error model.
Keywords: X scores and corresponding p-values derived using an error model
|
| |
|
| Overall design |
We profiled transcriptional responses upon the individual deletion of more than 260 TFs. We used a directed-weighted graph modelling approach and regulatory epistasis analysis to identify indirect regulatory relationships, and thus constructed a refined, functional transcriptional regulatory network.
|
| |
|
| Contributor(s) |
Hu Z, Killion PJ, Iyer VR |
| Citation(s) |
17417638 |
| |
| Submission date |
Apr 11, 2006 |
| Last update date |
Jul 31, 2013 |
| Contact name |
Vishy Iyer |
| E-mail(s) |
iyerlab@gmail.com
|
| Phone |
5122327833
|
| Organization name |
University of Texas at Austin
|
| Department |
Molecular Biosciences
|
| Street address |
2500 Speedway Dr. MBB 3.212
|
| City |
Austin |
| State/province |
TX |
| ZIP/Postal code |
78712 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL3588 |
UT Austin Iyer yeast 6.4k ORF-only cDNA platform |
|
| Samples (269)
|
|
| Relations |
| BioProject |
PRJNA95297 |
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