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Series GSE46707 Query DataSets for GSE46707
Status Public on Jul 06, 2014
Title Co-regulated gene expression downstream of a single transcription factor controls the acquisition of corticospinal motor neuron identity
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The neocortex contains an unparalleled diversity of neuronal subtypes responsible for complex behavior. Each cortical neuron has distinct traits, which are developmentally acquired under the control of batteries of neuron subtype-specific and pan-neuronal genes. The cis-regulatory logic that orchestrates the coordinated regulation of each unique combination of genes is not known for any class of neurons of the neocortex. We report that Fezf2, a transcription factor able to induce defining features of corticospinal motor neurons (CSMN), associates with the proximal promoters and regulates expression of series of CSMN signature genes. Fezf2 targets are functionally relevant as demonstrated by the finding that Fezf2 governs expression of the axon guidance receptor EphB1 to execute the ipsilateral extension of the corticospinal tract. Our data indicate that co-regulated expression of neuron subtype-specific gene batteries by a common transcription factor is one component of the regulatory logic responsible for the establishment of CSMN identity.
Overall design Fezf2 binding is characterized in neurospheres using ChIP-seq targeted against 3Flag-tagged Fezf2. Two types of control experiments were performed: mock IP and GFP-3Flag ChIP-seq. All experiments were performed in biological duplicate.
Contributor(s) Lodato S, Molyneaux BJ, Zuccaro E, Meleski A, Takahashi E, Mahony S, Gifford DK, Arlotta P
Citation(s) 24997765
Submission date May 07, 2013
Last update date May 15, 2019
Contact name Shaun Mahony
Phone 814-865-3008
Organization name Penn State University
Department Biochemistry & Molecular Biology
Lab Shaun Mahony
Street address 404 South Frear Bldg
City University Park
State/province PA
ZIP/Postal code 16802
Country USA
Platforms (2)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (6)
GSM1135048 ChIP-seq induced Fezf2-3Flag in neurospheres replicate 1
GSM1135049 ChIP-seq induced Fezf2-3Flag in neurospheres replicate 2
GSM1135050 ChIP-seq induced GFP-3Flag in neurospheres replicate 1
SRA SRP022156
BioProject PRJNA202295

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GSE46707_RAW.tar 1.4 Gb (http)(custom) TAR (of BED, BW, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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