GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE47084 Query DataSets for GSE47084
Status Public on Jan 01, 2015
Title Scl specifies hemogenic endothelium and inhibits cardiogenesis via primed enhancers [expression]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Scl/Tal1 confers hemogenic competence and prevents cardiomyogenesis in embryonic endothelium. Here we show that Scl both directly activates a broad gene regulatory network required for hematopoietic stem/progenitor cell (HS/PC) development, and represses transcriptional regulators required for cardiogenesis. Cardiac repression occurs during a short developmental window through Scl binding to distant cardiac enhancers that harbor H3K4me1 at this stage. Scl binding to hematopoietic regulators extends throughout HS/PC and erythroid development and spreads from distant enhancers to promoters. Surprisingly, Scl complex partners Gata 1 and 2 are dispensable for hematopoietic versus cardiac specification and Scl binding to the majority of its target genes. Nevertheless, Gata factors co-operate with Scl to activate selected transcription factors to facilitate HS/PC emergence from hemogenic endothelium. These results uncover a dual function for Scl in dictating hematopoietic versus cardiac fate choice and suggest a mechanism by which lineage-specific bHLH factors direct the divergence of competing fates.
Overall design Examination of Scl target genes in ES cell derived day4 EB (embryoid body) Flk+ mesodermal cells
Contributor(s) Org T, Duan D, Ferrari R, Montel-Hagen A, Van Handel B, Kerényi MA, Sasidharan R, Rubbi L, Fujiwara Y, Pellegrini M, Orkin SH, Kurdistani SK, Mikkola HK
Citation(s) 25564442
Submission date May 19, 2013
Last update date Feb 11, 2019
Contact name Tõnis Org
Phone 372-52-11484
Organization name University of Tartu
Department Institute of Molecular and Cell Biology
Lab Biotechnology
Street address Riia 23
City Tartu
ZIP/Postal code 51010
Country Estonia
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM1144560 WT_Flk+Scl+_rep1
GSM1144561 WT_Flk+Scl+_rep2
GSM1144562 WT_Flk+Scl-_rep1
This SubSeries is part of SuperSeries:
GSE47085 Scl specifies hemogenic endothelium and inhibits cardiogenesis via primed enhancers
BioProject PRJNA203511

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE47084_RAW.tar 20.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap