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Status |
Public on Aug 07, 2013 |
Title |
H. salinarum TrmB dynamics |
Organism |
Halobacterium salinarum NRC-1 |
Experiment type |
Other
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Summary |
Previous work characterized TrmB as a global glucose responsive metabolic transcription factor in archaeal extremophiles. However, it remains unclear how TrmB dynamically regulates its ~100 metabolic enzyme-coding genes. Using a dynamic perturbation approach, we elucidate the topology of the metabolic GRN in Halobacterium salinarum. We assayed gene expression in a wild-type and trmB knockout strain before and immedeatly following glucose perturbation. Clustering dynamic gene expression patterns reveals that TrmB functions alone to regulate central metabolic enzyme-coding genes, but cooperates with various regulators to control peripheral metabolic pathways.
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Overall design |
Gene expression of 100 genes was assayed in two strains - a wild-type and a trmB knockout. Two biological replicates of each strain were performed. Gene expression was assayed 240, 60, and 0 minutes prior to glucose addition and 5,10,20,45,90,180,360 minutes after.
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Contributor(s) |
Todor H, Schmid A |
Citation(s) |
23892291 |
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Submission date |
May 22, 2013 |
Last update date |
Aug 07, 2013 |
Contact name |
Horia Todor |
E-mail(s) |
horia.todor@duke.edu
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Organization name |
Duke University
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Department |
Biology
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Lab |
Schmid
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Street address |
125 Science Drive
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City |
Durham |
State/province |
North Carolina |
ZIP/Postal code |
27708 |
Country |
USA |
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Platforms (1) |
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Samples (48)
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Relations |
BioProject |
PRJNA205188 |
Supplementary file |
Size |
Download |
File type/resource |
GSE47201_raw_further-processed_data.xls.gz |
294.0 Kb |
(ftp)(http) |
XLS |
Processed data included within Sample table |
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