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| Status |
Public on Sep 15, 2013 |
| Title |
Quantitative transcriptional changes associated with chlorosis severity in mosaic leaves of tobacco plants infected with the Cucumber mosaic virus |
| Organism |
Nicotiana tabacum |
| Experiment type |
Expression profiling by array
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| Summary |
In this study, we compared transcriptomes in chlorotic tissues infected with wild type CMV or two CP mutants (129A and 129Q) with diverse chlorosis severity. Differentially expressed genes analysis showed that the CMV inoculation appeared to have similar effects on transcriptional expression profiles of the symptomatic chlorotic tissues and only the magnitude of expression differed among CMVs. Gene ontology analysis with biological process and cellular component terms revealed that many nuclear genes related to abiotic stress responses, including cadmium, heat, cold, and salt, were up-regulated while chloroplast- and photosynthesis-related genes (CPRGs) were down-regulated in the chlorotic tissues. Interestingly, down-regulation level of CPRGs was correlated with the chlorosis severity.
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| Overall design |
The expression difference genes (DEGs) between mock-inoculated healthy tissues and CMV-inoculated chlorotic tissues in mosaic leaves of tobacco plants were examined by the comparison on one-colour microarray data. Three biological replicates per each treatment were used in this experiment.
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| Contributor(s) |
Mochizuki T, Ohki ST |
| Citation(s) |
24745045 |
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Mochizuki, T., Ogata, Y., Hirata, Y. and Ohki, S. T. (2014), Quantitative transcriptional changes associated with chlorosis severity in mosaic leaves of tobacco plants infected with Cucumber mosaic virus. Molecular Plant Pathology, 15: 242-254. doi: 10.1111/mpp.12081
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| Submission date |
May 28, 2013 |
| Last update date |
Nov 18, 2015 |
| Contact name |
Tomofumi MOCHIZUKI |
| E-mail(s) |
tomochi@plant.osakafu-u.ac.jp
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| Organization name |
Osaka Prefecture University
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| Department |
Graduate School of Life and Environmental Sciences
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| Street address |
1-1, Gakuen-cho, Naka-ku
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| City |
Sakai |
| State/province |
Osaka |
| ZIP/Postal code |
599-8531 |
| Country |
Japan |
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| Platforms (1) |
| GPL10098 |
Agilent-021113 Tobacco Gene Expression Microarray (Feature Number version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA205533 |
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