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| Status |
Public on Sep 01, 2013 |
| Title |
Analysis of change in circulating microRNAs in mice during breast tumor development |
| Platform organism |
Homo sapiens |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
The aim of this study was to analyse alterations in circulating microRNA expression during breast tumour progression in a murine model, and to apply significantly altered miRNAs to patient samples to identify novel circulating microRNA markers or tumour progression. Athymic nude mice (n=10) received an injection of 2 x 105 MDA-MB-231 cells. Tumour volume was monitored weekly and blood sampling performed at weeks 1 and 6 following tumour induction. A microRNA array was performed comparing circulating 384 microRNAs in animals with early (week 1, n=5) versus late (week 6, n=5) disease.
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| Overall design |
Five animals were included in the study (n=2 samples from each, total n=10 microArrays performed), with whole blood sampled one week following tumor induction and again at 6 weeks in the presence of established disease. The change in microRNA profile was then compared at week 1 and week 6, which the "early" sample providing the baseline measurements for each animal.
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| Contributor(s) |
Dwyer RM |
| Citation(s) |
24626163 |
| |
| Submission date |
May 29, 2013 |
| Last update date |
Sep 08, 2014 |
| Contact name |
Róisín M Dwyer |
| Organization name |
National University of Ireland Galway
|
| Street address |
Costelloe Road
|
| City |
Galway |
| ZIP/Postal code |
Galway |
| Country |
Ireland |
| |
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| Platforms (1) |
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| Samples (10)
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| GSM1149898 |
Blood_tumour induction_week1 [SAMPLE 101] |
| GSM1149899 |
Blood_tumour induction_week6 [SAMPLE 106] |
| GSM1149900 |
Blood_tumour induction_week1 [SAMPLE 201] |
| GSM1149901 |
Blood_tumour induction_week6 [SAMPLE 206] |
| GSM1149902 |
Blood_tumour induction_week1 [SAMPLE 301] |
| GSM1149903 |
Blood_tumour induction_week6 [SAMPLE 306] |
| GSM1149904 |
Blood_tumour induction_week1 [SAMPLE 401] |
| GSM1149905 |
Blood_tumour induction_week6 [SAMPLE 406] |
| GSM1149906 |
Blood_tumour induction_week1 [SAMPLE 501] |
| GSM1149907 |
Blood_tumour induction_week6 [SAMPLE 506] |
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| Relations |
| BioProject |
PRJNA205636 |
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