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| Status |
Public on Dec 31, 2015 |
| Title |
Gene expression changes in Apc-mutant mouse intestinal organoids 7 and 20 days after the induction of the Apc deletion |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We isolated and selected intestinal adenoma organoids from Lgr5-EGFP-IRES-CreER; Apcflox/flox mice and added tamoxifen to induce the deletion of the Apc gene in the intestinal stem cells. Gene expressions on day7 and day20 after the addition of tamoxifen were compared, representing two stages with different colorectal cancer stem cell content.
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| Overall design |
Total RNA obtained from Lgr5-EGFP-IRES-CreER; Apcflox/flox organoids were compared 7 days and 20 days after the addition of tamoxifen, cultured without the Wnt-agonist R-Spondin1.
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| Contributor(s) |
Wiener Z, Hogstrom J, Hyvonen V, Alitalo K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 31, 2013 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Zoltan Wiener |
| E-mail(s) |
zoltan.wiener@helsinki.fi
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| Organization name |
University of Helsinki
|
| Department |
Molecular Cancer Biology Programme
|
| Street address |
Haartmaninkatu 8
|
| City |
Helsinki |
| ZIP/Postal code |
FI-00017 |
| Country |
Finland |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA206080 |
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