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| Status |
Public on Jul 01, 2014 |
| Title |
Ozone Exposure Alters MicroRNA Expression Profiles in Humans |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
We set out to test the hypothesis that ozone inhalation exposure significantly alters miRNA expression profiles within the airway of humans. Adult human volunteers were exposed to 0.4 ppm ozone for two hours. Induced sputum samples were collected from each subject 48 hours pre-exposure and 6 hours post-exposure. Genome-wide miRNA expression profiles were evaluated using microarray analysis.
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| Overall design |
Twenty healthy non-asthmatic adult subjects aged 18 – 37 years, with no history of smoking in the past 10 years, completed the study. The ozone exposures were conducted in an exposure chamber. Each subject was exposed to 0.4 ppm ozone for two hours while performing four 15-minute sessions of intermittent moderate exercise (expiratory minute ventilation, 30–40 L/min) on a treadmill, separated by 15 minutes of seated rest. Induced sputum samples were collected from each subject 48 hours pre-exposure and 6 hours post-exposure. RNA samples were homogenized in TRIzol, and extracted according to standard TRIzol protocol. RNA was labeled and hybridized to the Agilent Human miRNA Microarray (v1.0).
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| Contributor(s) |
Fry RC, Rager JE, Jaspers I, Alexis NE |
| Citation(s) |
24771714 |
| |
| Submission date |
Jun 14, 2013 |
| Last update date |
Jul 02, 2014 |
| Contact name |
Rebecca Catherine Fry |
| E-mail(s) |
rfry@unc.edu
|
| Organization name |
UNC-Chapel Hill
|
| Department |
Environmental Sciences and Engineering
|
| Street address |
1213 MHRC
|
| City |
Chapel Hill |
| ZIP/Postal code |
27599 |
| Country |
USA |
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| Platforms (1) |
| GPL6955 |
Agilent-016436 Human miRNA Microarray 1.0 (Feature Number version) |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA208519 |
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