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Series GSE48132 Query DataSets for GSE48132
Status Public on Jan 01, 2014
Title Aryl hydrocarbon receptor senses bacterial pigmented virulence factors and orchestrates anti-bacterial defenses (part 2)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The aryl hydrocarbon receptor (AhR) is a highly conserved ligand-dependent transcription factor that senses environmental toxins and endogenous ligands, thereby inducing detoxifying enzymes and modulating immune cell differentiation and responses. We hypothesized that AhR not only evolved to sense pollutants from the environment, but also insults of microbial origin. We demonstrate that bacterial pigmented virulence factors, namely the phenazines pyocyanin and 1-hydroxyphenazine from Pseudomonas aeruginosa and the naphthoquinone phthiocol from Mycobacterium tuberculosis, are ligands of AhR. AhR activation leads to degradation of these virulence factors and regulated cytokine and chemokine production. The relevance of AhR to host defense is underlined by heightened susceptibility of AhR-deficient mice, both to P. aeruginosa and M. tuberculosis. Our data demonstrate that AhR senses distinct bacterial virulence factors and controls anti-bacterial responses. We provide evidence for a previously unidentified role for AhR as an intracellular pattern recognition receptor, and identify bacterial pigmented virulence factors as a new class of pathogen-associated molecular patterns.
 
Overall design Microarray experiments were performed as dual-color hybridizations. To compensate for dye-specific effects, a dye-reversal color-swap was applied. Quality control and quantification of total RNA amount was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies) and a NanoDrop 1000 spectrophotometer (Kisker).
 
Contributor(s) Moura-Alves P, Houthuys E, Faé K, Dorhoi A, Kaufmann SH, Mollenkopf HJ
Citation(s)
  • Moura-Alves P, Faé K, Houthuys E, Dorhoi A et al. AhR sensing of bacterial pigments regulates antibacterial defence. Nature 2014 Aug 28;512(7515):387-92. PMID: 25119038
Submission date Jun 19, 2013
Last update date Feb 22, 2018
Contact name Hans-Joachim Mollenkopf
E-mail(s) mollenkopf@mpiib-berlin.mpg.de
Phone +49 30 28460 482
Organization name Max-Planck-Institute for Infection Biology
Lab Microarray/Genomics Core Facility
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (12)
GSM1169195 scr 24h DMSO_1 vs. scr 24h pyo_2
GSM1169196 scr 24h pyo_1 vs. scr 24h DMSO_2
GSM1169197 scr 24h DMSO_1 vs. scr 24h hp_2
This SubSeries is part of SuperSeries:
GSE48133 Aryl hydrocarbon receptor senses bacterial pigmented virulence factors and orchestrates anti-bacterial defenses
Relations
BioProject PRJNA209088

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE48132_RAW.tar 193.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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