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| Status |
Public on Dec 11, 2013 |
| Title |
Real- time quantiative PCR analysis of HSC derived from sleep deprived mice |
| Platform organisms |
Mus musculus; Rattus norvegicus |
| Sample organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
HSC were derived from mice sleep deprived for 4 hours and compared to mice that slept for 4 hours during the same circadian time. 5 mice were pooled for each sample and bones from femur, tibia and spine were crushed and stained to lineage deplete and enrich for ckit+, sca-1+,Flk-,CD150+, CD34-
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| Overall design |
qpcr miRNA profiling from approximately 50ng total RNA extracted from HSC FACS sorted into Trizol.
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| Contributor(s) |
Ibarra I, Rolls A |
| Citation(s) |
26465715 |
| Submission date |
Jun 20, 2013 |
| Last update date |
Oct 29, 2019 |
| Contact name |
Irv Weissman |
| Organization name |
Stanford University
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| Department |
Stem Cell Institute
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| Lab |
Weissman Lab
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| Street address |
265 Campus Dr. West
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL14646 |
TaqMan Rodent MicroRNA A Array v2.0 |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA208998 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE48144_fold_change.txt.gz |
3.0 Kb |
(ftp)(http) |
TXT |
| GSE48144_non-normalized_matrix.txt.gz |
3.7 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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