|
| Status |
Public on Oct 01, 2007 |
| Title |
Broad, ectopic expression of the sperm protein, PLCZ1 induces parthenogenesis and ovarian tumours in mice |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Mammalian metaphase II (mII) exit and embryogenesis are induced at fertilization by a signal thought to come from the sperm protein, phospholipase C-zeta (PLCZ1). Meiotic progression can also be triggered without sperm, as in parthenogenesis, although the classic mouse in vivo parthenogenetic model, LT/Sv, fails in meiosis I due to an unknown molecular etiology. We here dissect PLCZ1 specificity and function in vivo and address its ability to interfere with maternal meiotic exit. Wild-type mouse Plcz1 expression was restricted to post-pubertal testes, and the brains of both sexes, with region-specifying elements mapping to a 4.1 kb Plcz1 promoter fragment. When broad ectopic PLCZ1 expression was forced in independent transgenic lines, they initially appeared healthy. Their oocytes underwent unperturbed meiotic maturation to mII but subsequently exhibited autonomous intracellular free calcium oscillations, second polar body extrusion, pronucleus formation and parthenogenetic development. Transfer of transgenic cumulus cell nuclei into wild-type oocytes induced activation and development, demonstrating a direct effect of PLCZ1 analogous to fertilization. Whilst Plcz1 transgenic males remained largely asymptomatic, females developed abdominal swellings caused by benign ovarian teratomas that were under-represented for paternally- and placentally-expressed transcripts. Plcz1 was not over-expressed in the ovaries of LT/Sv or in human germline ovarian tumours. The narrow spectrum of PLCZ1 activity indicates that it is modulated by tissue-restricted accessory factors. This work characterizes a novel model in which parthenogenesis and tumourigenesis follow full meiotic maturation and are linked to fertilization by PLCZ1.
Keywords: miRNA profiling; expression profiling of mouse miRNAs_SampleB
|
| |
|
| Overall design |
Fourteen samples were analyzed for the study.
|
| |
|
| Contributor(s) |
Perry A |
| Citation(s) |
17933795 |
| |
| Submission date |
May 12, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Anthony CF Perry |
| E-mail(s) |
perry135@aol.com
|
| Phone |
+81 78 306 3054
|
| Fax |
+81 78 306 3144
|
| Organization name |
RIKEN
|
| Department |
Center for Developmental Biology
|
| Lab |
Laboratory of Mammalian Molecular Embryology
|
| Street address |
2-2-3 Minatojima-minamimachi
|
| City |
Kobe |
| State/province |
Hyogo-ken |
| ZIP/Postal code |
650-0047 |
| Country |
Japan |
| |
|
| Platforms (3) |
| GPL3754 |
Riken Mouse 4K miRNA array M8.0 |
| GPL5938 |
Riken Mouse 4K miRNA array M8.1 |
| GPL5939 |
Riken Mouse 4K miRNA array M8.2 |
|
| Samples (14)
|
|
| Relations |
| BioProject |
PRJNA95631 |
Less...
More...