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Series GSE4822 Query DataSets for GSE4822
Status Public on Oct 01, 2007
Title Broad, ectopic expression of the sperm protein, PLCZ1 induces parthenogenesis and ovarian tumours in mice
Organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary Mammalian metaphase II (mII) exit and embryogenesis are induced at fertilization by a signal thought to come from the sperm protein, phospholipase C-zeta (PLCZ1). Meiotic progression can also be triggered without sperm, as in parthenogenesis, although the classic mouse in vivo parthenogenetic model, LT/Sv, fails in meiosis I due to an unknown molecular etiology. We here dissect PLCZ1 specificity and function in vivo and address its ability to interfere with maternal meiotic exit. Wild-type mouse Plcz1 expression was restricted to post-pubertal testes, and the brains of both sexes, with region-specifying elements mapping to a 4.1 kb Plcz1 promoter fragment. When broad ectopic PLCZ1 expression was forced in independent transgenic lines, they initially appeared healthy. Their oocytes underwent unperturbed meiotic maturation to mII but subsequently exhibited autonomous intracellular free calcium oscillations, second polar body extrusion, pronucleus formation and parthenogenetic development. Transfer of transgenic cumulus cell nuclei into wild-type oocytes induced activation and development, demonstrating a direct effect of PLCZ1 analogous to fertilization. Whilst Plcz1 transgenic males remained largely asymptomatic, females developed abdominal swellings caused by benign ovarian teratomas that were under-represented for paternally- and placentally-expressed transcripts. Plcz1 was not over-expressed in the ovaries of LT/Sv or in human germline ovarian tumours. The narrow spectrum of PLCZ1 activity indicates that it is modulated by tissue-restricted accessory factors. This work characterizes a novel model in which parthenogenesis and tumourigenesis follow full meiotic maturation and are linked to fertilization by PLCZ1.
Keywords: miRNA profiling; expression profiling of mouse miRNAs_SampleB
Overall design Fourteen samples were analyzed for the study.
Contributor(s) Perry A
Citation(s) 17933795
Submission date May 12, 2006
Last update date Mar 16, 2012
Contact name Anthony CF Perry
Phone +81 78 306 3054
Fax +81 78 306 3144
Organization name RIKEN
Department Center for Developmental Biology
Lab Laboratory of Mammalian Molecular Embryology
Street address 2-2-3 Minatojima-minamimachi
City Kobe
State/province Hyogo-ken
ZIP/Postal code 650-0047
Country Japan
Platforms (3)
GPL3754 Riken Mouse 4K miRNA array M8.0
GPL5938 Riken Mouse 4K miRNA array M8.1
GPL5939 Riken Mouse 4K miRNA array M8.2
Samples (14)
GSM232180 wt ovary
GSM232181 wt placenta
GSM232182 CV885
BioProject PRJNA95631

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE4822_Averaged_normalized_data.txt 28.9 Kb (ftp)(http) TXT
GSE4822_ClusterGraph.txt 54.8 Kb (ftp)(http) TXT
GSE4822_RAW.tar 940.0 Kb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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