|
| Status |
Public on Jun 29, 2013 |
| Title |
Determination of Sigma 54 reglons in Bacillus thuringiensis HD73 strain |
| Organism |
Bacillus thuringiensis |
| Experiment type |
Expression profiling by array
|
| Summary |
To determine Sigma 54 (SigL) reglons in Bacillus thuringiensis HD73 strain, A sigLmutant, HD(ΔsigL::kan), was constructed with insertion of kanamycin resistance gene cassete. We have employed whole genome microarray expression profiling as a discovery platform to identify the difference of gene expression between mutant and wild-type strains.
|
| |
|
| Overall design |
2 ml samples were separately harvested from B. thuringiensis HD73 and HD(ΔsigL::kan) strains grown in Schaeffer’s sporulation medium (SSM) at stages T7 of stationary phase (7 hours after the end of the exponential phase). Three independent repeats were performed for each stain.
|
| |
|
| Contributor(s) |
Song F, Peng Q |
| Citation(s) |
27404799, 38605001 |
| |
| Submission date |
Jun 28, 2013 |
| Last update date |
Apr 16, 2024 |
| Contact name |
Fuping Song |
| E-mail(s) |
fpsong@ippcaas.cn
|
| Phone |
0086-10-62896634
|
| Organization name |
Instititue of plant protection, CAAS
|
| Street address |
No 2 West Yuan-Ming-Yang Road
|
| City |
Beijing |
| ZIP/Postal code |
1000193 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL17384 |
Agilent-037484 suyunjin 037483 (Probe Name version) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA209986 |
Less...
More...