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| Status |
Public on Jul 17, 2013 |
| Title |
Expression data from tissues during somatic embryogenesis in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
We collected tissues from bent cotyledon stage zygotic embryos, proliferating tissue at day 7 and day 14 induction of somatic embryogenesis and mature somatic emrbyos in a wild type (Col-0) and vtc2 (SALK_146824) insertion.
We used microarrays to identify global patterns of gene activity during somatic embryogenesis in a wild type (Col-0) and vitamin C deficient mutant (vtc2)
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| Overall design |
RNA was extracted and amplified from four stages of somatic embryogenesis (bent cotyledon, day 7 induction, day 14 induction, and mature somatic embryos) in a wild type (Col-0) and vitamin C deficient mutant (vtc2) before being hybridized to the Arabidopsis ATH1 GeneChip in duplicate (two biological replicates).
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| Contributor(s) |
Belmonte M, Chan A, Becker M, Mao S, Lee S |
| Citation(s) |
25151615 |
| Submission date |
Jul 16, 2013 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Mark Findlay Belmonte |
| Organization name |
University of Manitoba
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| Department |
Biological Sciences
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| Lab |
Belmonte
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| Street address |
50 Sifton Road
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| City |
Winnipeg |
| State/province |
Manitoba |
| ZIP/Postal code |
R3T 2N2 |
| Country |
Canada |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (16)
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| GSM1186749 |
wild type day 7 induction, bio rep2 |
| GSM1186750 |
wild type day 14 induction, bio rep1 |
| GSM1186751 |
wild type day 14 induction, bio rep2 |
| GSM1186752 |
wild type mature somatic embryo, bio rep1 |
| GSM1186753 |
wild type mature somatic embryo, bio rep2 |
| GSM1186754 |
vtc2 zygotic embryo, bio rep1 |
| GSM1186755 |
vtc2 zygotic embryo, bio rep2 |
| GSM1186756 |
vtc2 day 7 induction, bio rep1 |
| GSM1186757 |
vtc2 day 7 induction, bio rep2 |
| GSM1186758 |
vtc2 day 14 induction, bio rep1 |
| GSM1186759 |
vtc2 day 14 induction, bio rep2 |
| GSM1186760 |
vtc2 mature somatic embryo, bio rep1 |
| GSM1186761 |
vtc2 mature somatic embryo, bio rep2 |
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA212312 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE48915_RAW.tar |
36.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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