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| Status |
Public on Sep 01, 2013 |
| Title |
TALEN-Engineered AR Gene Rearrangements Reveal Endocrine Uncoupling of Androgen Receptor in Prostate Cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
This experiment addressed the question of whether full length Androgen receptor and Androgen Receptor variant ARv567es have similar transciptional activities. The transcriptomes of R1-AD1, a AR full length expressing cell line, are compared in an AR on (1nM DHT) to an AR off (ethanol vehicle) state. R1-D567 was derived from R1-AD1 and modified with TALENs in such a way as to express only AR-V567es. The AR on and off states were induced with a control siRNA (AR on) or a siRNA targeting exon 1 (AR off).
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| Overall design |
R-AD1 was cultured for 24 hours in either base RPMI media with 1nM DHT or ethanol vehichle and RNA extracted. R1-D567 cells were electroporated with control siRNA or siRNA targeting exon 1 of AR and allowed to recover 48 hours and then cultured in base media with 1nM DHT or ethanol for 24 hrs. Each conditon was done in triplicate.
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| |
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| Contributor(s) |
Nyquist MD, Dehm SM |
| Citation(s) |
24101480 |
| |
| Submission date |
Jul 25, 2013 |
| Last update date |
Jan 28, 2016 |
| Contact name |
Michael David Nyquist |
| E-mail(s) |
micknyquist@gmail.com
|
| Phone |
612-961-6367
|
| Organization name |
University of Minnesota
|
| Department |
Genetics Cell Developmental Bio
|
| Lab |
Scott Dehm and Dan Voytas
|
| Street address |
Masonic Cancer Center, University of Minnesota, Mayo Mail Code 806 420 Delaware St SE
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL10904 |
Illumina HumanHT-12 V4.0 expression beadchip (gene symbol) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA213269 |
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