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| Status |
Public on Aug 13, 2013 |
| Title |
The role of the Cj0440c gene in Campylobacter jejuni |
| Organism |
Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 |
| Experiment type |
Expression profiling by array
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| Summary |
Cj0440c encodes a putative transcriptional regulator. To determine the role of Cj0440c in C.jejuni, we knocked out Cj0440c in the wild-type strain (S) to obtain the Cj0440c mutants (SM). Then we compared the transcriptome of the Cj0440c mutant with that of the parent strain using DNA microarray. These comparisons identified 19 genes that showed a≥2-fold change in expression in SM. The differentially expressed genes in SM encode proteins involved in flagellar biosynthesis, O-linked glycosylation and hypothetical proteins with unknown fuctions. Cj0440c may regulate flagellar structural element expression or as a compenent of flagellar complex co-expressed with other flagellar genes. Subsequent experiments demonstrated that inactivation of Cj0440c affected corresponding phenotypes of C.jejuni, including broken flagella, weaker motility and reduced colonization ability in chickens.
These findings indicate that Cj0440c governs the expression of multiple genes related to flagellar biosynthesis and O-linked glycosylation. This study provides favorable evidence for completing the information of the Campylobacter jejuni genome.
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| Overall design |
An eight chip study using total RNA recoverd from four separate wild-type cultures of Campylobacter jejuni NCTC111168 (S) and four separate cultures of a mutant strain, Campylobacter jejuni NCTC11168 delta- Cj0440c (SM), in which Cj0440c is deleted. Each chip measures the expression level of 1634 genes from Campylobacter jejuni NCTC11168.
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| Contributor(s) |
Fang X, Hao H, Yuan Z |
| Citation missing |
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| Submission date |
Jul 26, 2013 |
| Last update date |
Aug 15, 2013 |
| Contact name |
Xia Fang |
| E-mail(s) |
fangxia1007@163.com
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| Phone |
027-87287140
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| Organization name |
Huazhong Agricultural University
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| Lab |
National Reference Laboratory of Veterinary Drug Residues
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| Street address |
Shizishan Street
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| City |
wuhan |
| State/province |
hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
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| Platforms (1) |
| GPL17410 |
NimbleGen Campylobacter jejuni NCTC11168 Whole-Genome microarray [080328_C_jejuni_NCTC_11168_expr] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA214940 |
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