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| Status |
Public on Jul 31, 2013 |
| Title |
Medaka larvae were exposed to HBCD |
| Platform organism |
Oryzias latipes |
| Sample organism |
Oryzias melastigma |
| Experiment type |
Expression profiling by array
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| Summary |
Recently, omics techniques have been widely applied to the discovery of potential bio-markers and explore triggering mechanism. To get a more comprehensive diagnosis of HBCD impacts on marine medaka (Oryzias melastigma), the larvae (within 24 hours post-hatch) were exposed to gradient doses of HBCD. After exposure for 7 days, the profiles of genes expression were examined using a custom-commercial 26, 430-oligonucleotide arrays (4×44K) of Japanese medaka which is shared much genomic information with marine medaka.At the end of the treatment period, 30 larvae/sample were pooled for RNA extraction and labeled by One-Color. A total of twelve independent arrays: three control (DMSO), three low-concentration HBCD (0.2 nM) exposures, three medium-concentration HBCD (2 nM) exposures, and three high-concentration HBCD (20 nM) exposures.
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| Overall design |
The larvae of marine medaka (within 24 hours post-hatch) were exposed to to 0 (control), 0.2nM, 2nM and 20nM of HBCD (dimethyl sulfoxide with a final concentration of 1:30000 v/v water) for 7 days. Each HBCD treatment had three replicates with 100 larvae for each Petri dish. At the end of the treatment period, 30 larvae/sample were pooled for RNA extraction. A total of twelve independent arrays: three control (DMSO), three low-concentration HBCD (0.2 nM) exposures, three medium-concentration HBCD (2 nM) exposures, and three high-concentration HBCD (20 nM) exposures.
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| Contributor(s) |
Zuo Z, Geng H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jul 30, 2013 |
| Last update date |
Aug 02, 2013 |
| Contact name |
geng hong |
| E-mail(s) |
kaixin522116@163.com
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| Phone |
13616057613
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| Organization name |
School of life sciences
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| Department |
Biology
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| Lab |
Toxicology
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| Street address |
School of Life Sciences, Xiamen University,
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| City |
xiamen city |
| ZIP/Postal code |
361005 |
| Country |
China |
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| Platforms (1) |
| GPL17510 |
Agilent-023673 Custom Oryzias latipes (Japanese medaka) 4x44K microarray |
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| Samples (12)
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| GSM1198127 |
Oryzias_melastigma_Larvae_7days_Control_0_rep1 |
| GSM1198128 |
Oryzias_melastigma_Larvae_7days_Control_0_rep2 |
| GSM1198129 |
Oryzias_melastigma_Larvae_7days_Control_0_rep3 |
| GSM1198130 |
Oryzias_melastigma_Larvae_7days_Low_0.2_rep1 |
| GSM1198131 |
Oryzias_melastigma_Larvae_7days_Low_0.2_rep2 |
| GSM1198132 |
Oryzias_melastigma_Larvae_7days_Low_0.2_rep3 |
| GSM1198133 |
Oryzias_melastigma_Larvae_7days_Medium_2_rep1 |
| GSM1198134 |
Oryzias_melastigma_Larvae_7days_Medium_2_rep2 |
| GSM1198135 |
Oryzias_melastigma_Larvae_7days_Medium_2_rep3 |
| GSM1198136 |
Oryzias_melastigma_Larvae_7days_High_20_rep1 |
| GSM1198137 |
Oryzias_melastigma_Larvae_7days_High_20_rep2 |
| GSM1198138 |
Oryzias_melastigma_Larvae_7days_High_20_rep3 |
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| Relations |
| BioProject |
PRJNA213726 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE49362_RAW.tar |
24.9 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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