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Series GSE4971 Query DataSets for GSE4971
Status Public on May 20, 2007
Title Sugarcane transcriptome - Drought response
Platform organism Saccharum officinarum
Sample organism Saccharum hybrid cultivar SP90-1638
Experiment type Expression profiling by array
Summary Sugarcane one-eyed seed sets obtained from a variety sensitive to drought conditions (cv. SP90-1638, CTC, Brazil) were cultivated on moist sand for 15 days. The plants were transferred to pots containing moist sand and were irrigated with Hoagland’s solution (Hoagland and Arnon, 1950). Regular watering was maintained for 90 days and suppressed after this period for the experimental group. Leaves were collected 24 h, 72 h and 120 h after exposure to drought conditions for the control and experimental groups. Samples were collected and immediately frozen in liquid nitrogen. Leaves from six plantlets were used for each time point. Extraction of total RNA was performed separately on each sample pool.
Keywords: time course of stress response
 
Overall design RNA from experimental sample and from its respective control sample (untreated plants) was used for hybridization to dual channel arrays. Two biological replicates were used for each experimental point. The quantification of each hybridization was submitted in two files, one for each slide side (technical replicates).
 
Contributor(s) Rodrigues FA, di Mauro SZ, Rocha FR, Papini-Terzi FS, Nishiyama-Jr MY, Vêncio RZ, Vicentini R, Souza GM
Citation(s) 17355627, 19302712
Submission date Jun 01, 2006
Last update date Aug 27, 2019
Contact name Milton Yutaka Nishiyama Jr
E-mail(s) yutakajr@gmail.com
URL http://sucest-fun.org
Organization name Universidade de São Paulo - USP
Department Biochemistry
Lab Signal Transduction Laboratory
Street address Av. Professor Lineu Prestes, 748
City São Paulo
State/province São Paulo
ZIP/Postal code 05508-000
Country Brazil
 
Platforms (1)
GPL3799 SUCAST Sugarcane 2208 v1.0
Samples (12)
GSM110514 Sugarcane transcriptome - ch2: Drought_24h_Exp_1; ch1: control_24h_1 (L)
GSM110515 Sugarcane transcriptome - ch2: Drought_24h_Exp_1; ch1: control_24h_1 (R)
GSM110516 Sugarcane transcriptome - ch1: Drought_24h_Exp_2; ch2: control_24h_2 (L)
This SubSeries is part of SuperSeries:
GSE14732 Sugarcane genes associated with sucrose content relate to drought and cell wall metabolism
Relations
BioProject PRJNA114679

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