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Status |
Public on Jan 21, 2014 |
Title |
Identification of Lgr5-independent spheroid-generating progenitors of the mouse fetal intestinal epithelium |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Immortal spheroids were generated from fetal mouse intestine using the culture system developed to culture organoids from adult intestinal epithelium. Spheroids are made of a monostratified polarized epithelium displaying a poorly differentiated intestinal phenotype. The proportion of spheroids generated from intestinal explants progressively decreases from fetal to postnatal period, with a corresponding increase in production of organoids. Spheroid cells show indefinite self-renewing properties but exhibit a transcriptome strikingly different from that of adult intestinal stem cells reminiscent of incompletely caudalized progenitors. The receptor Lgr4, but not Lgr5, is essential for their growth. Trop2/Tacstd2 and Cnx43/Gja1, two markers highly enriched in spheroids, are expressed throughout the E14 intestinal epithelium. Comparison of in utero and neonatal lineage tracing using Cnx43-CreER and Lgr5-CreERT2 mice identified spheroid-generating cells as developmental progenitors involved in generation of the prenatal intestinal epithelium. Ex vivo, spheroid cells have the potential to differentiate into organoids, thus qualifying them as a new type of intestinal stem-like cells.
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Overall design |
Two-channel microarray experiments were performed from spheroid/organoid pairs isolated each from a given embryo. Following initial seeding of small intestine from a given embryo/mouse (at E16, E18 or P0), spheroids and organoids were selectively picked up for each animal and replated for 3 passages to reach sample homogeneity. Hybridization was performed on the 4 independent pairs with dye-swap.
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Contributor(s) |
Mustata RC, Vasile G, Fernandez-Vallone V, Strollo S, Lefort A, Libert F, Monteyne D, Pérez-Morga D, Vassart G, Garcia M |
Citation(s) |
24139799 |
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Submission date |
Aug 12, 2013 |
Last update date |
Jan 21, 2014 |
Contact name |
Frederick Libert |
E-mail(s) |
flibert@ulb.ac.be
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Organization name |
ULB
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Department |
IRIBHM
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Lab |
C2-140
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Street address |
808 route de Lennik
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City |
Brussels |
ZIP/Postal code |
B-1070 |
Country |
Belgium |
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Platforms (1) |
GPL6943 |
ULB MEEBO Mouse 39K array version 1 |
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Samples (4)
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GSM1207259 |
Mouse Newborn intestinal spheroid/organoid pair _P0 |
GSM1207260 |
Mouse fetal intestinal spheroid/organoid pair _E16_1 |
GSM1207261 |
Mouse fetal intestinal spheroid/organoid pair _E16_2 |
GSM1207262 |
Mouse fetal intestinal spheroid/organoid pair _E18 |
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Relations |
BioProject |
PRJNA214963 |
Supplementary file |
Size |
Download |
File type/resource |
GSE49803_RAW.tar |
43.7 Mb |
(http)(custom) |
TAR (of GPR) |
Processed data included within Sample table |
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