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Series GSE49812 Query DataSets for GSE49812
Status Public on Aug 01, 2014
Title Expression profiles of Actinobacillus pleuropneumoniae 4074 and the qseB/qseC gene-deleted mutant
Organism Actinobacillus pleuropneumoniae
Experiment type Expression profiling by array
Summary To determine the role of Actinobacillus pleuropneumoniae two-component system QseBQseC, we constructed a qseBqseC gene-deleted mutant ΔqseBΔqseC based on the wild type A. pleuropneumoniae 4074. The transcriptional profiles were compared between the A. pleuropneumoniae ΔqseBΔqseC and its parental strain under the normal growth condition using microarray. A total of 44 genes were found differentially expressed (DE) compared to the wild type strain. These functional genes are primarily related to metabolism, cell wall biogenesis, energy, replication and recombination. Further investigations indicated that the type IV pili (Tfp) assembly protein PilM is regulated directly by QseB, and PilM is essential for adherence and virulence. Characterization of the QseBQseC regulon genes will provides new insight into understanding of the relevant signal transduction pathways and prevention of the infection.
 
Overall design A. pleuropneumoniae strains were cultured in TSB medium supplemented with 10 μg/ml of nicotinamide adenine dinucleotide (NAD) and 10% (v/v)filtered cattle serum at 37℃. The samples were collected at the mid-exponential phase and the total RNA were extracted using RNA-Solv Reagent (Omega) according to the manufacturer’s instructions. Expression profiles of two different Actinobacillus pleuropneumoniae (4074 and ΔqseBΔqseC) were determined. The fold changes >=1.5 or <=-1.5 were selected as differentially expressed genes.
 
Contributor(s) Liu J, Bei W
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Submission date Aug 12, 2013
Last update date Sep 03, 2014
Contact name Jinlin Liu
E-mail(s) liujinlin2009@126.com
Organization name Central China Normal University
Department College of Life Science
Street address 152# Luoyu Street
City Wuhan
ZIP/Postal code 430079
Country China
 
Platforms (1)
GPL9691 Agilent-019883 Actinobacillus pleuropneumoniae expression array
Samples (2)
GSM1207523 4074_normalized signal
GSM1207524 delta-qseB-delta-qseC_normalized signal
Relations
BioProject PRJNA214982

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE49812_RAW.tar 4.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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