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| Status |
Public on Dec 03, 2013 |
| Title |
Identification of Smaug target mRNAs in the early Drosophila embryo using RIP-Chip |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
To identify Smaug’s target mRNAs on a genome-wide scale we used ribonucleoprotein (RNP) co-immunoprecipitation followed by microarray analysis of the co-purifying mRNAs (RIP-Chip). Extracts, prepared from wild-type embryos collected 0-3 hours post-egglaying, were immunoprecipitated with an anti-Smaug antibody (Smaug RIPs) while control immunoprecipitations using non-immune serum served as a negative control (control RIPs). Genome-wide transcript expression in wild-type embryos were also assessed and used as reference.
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| Overall design |
There are 11 array experiments presented here: 1. gene expression profiling of total mRNA sample extracted from wild-type 0-3 hour embryos (3 technical replicates performed using a pooled input reference sample); 2. RNA co-immunoprecipitations of endogenous Smaug (Smaug RIPs) from wild-type 0-3 hour embryos (3 biological replicates and 1 technical replicates); 3. control RNA co-immunoprecipitations (control RIPs) from wild-type 0-3 hour embryos (3 biological replicates and 1 technical replicates).
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| Contributor(s) |
Chen L, Dumelie JG, Li X, Cheng MH, Yang Z, Laver JD, Siddiqui NU, Westwood JT, Morris QD, Lipshitz HD, Smibert CA |
| Citation(s) |
24393533 |
| |
| Submission date |
Aug 16, 2013 |
| Last update date |
Mar 04, 2014 |
| Contact name |
Howard D Lipshitz |
| E-mail(s) |
howard.lipshitz@utoronto.ca
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| Organization name |
University of Toronto
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| Department |
Molecular Genetics
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| Street address |
1 King's College Circle
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| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5S1A8 |
| Country |
Canada |
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| Platforms (1) |
| GPL13782 |
NimbleGen Drosophila melanogaster Gene expression array [DM4_3_60mer_expr] |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA215420 |
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