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| Status |
Public on Oct 31, 2013 |
| Title |
The transcriptomic response of rat hepatic stellate cells to endotoxin: implications for hepatic inflammation and immune regulation |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
Located in the perisinusoidal space of Disse, hepatic stellate cells (HSCs) communicate with all other liver cell types by physical association and / or by producing cytokines and chemokines. In liver disease and folllowing liver transplantation, elevated levels of endotoxin (bacterial lipopolysaccharide: LPS) stimulate HSCs to produce increased amounts of cytokines and chemokines. Transcriptomic analysis of cultured HSCs stimulated with LPS yields a survey of expression changes which potentially modulate the hepatic inflammatory and immune responses.
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| Overall design |
In two independent experiments, rat HSCs were cultured with or without 10 ng/ml LPS for 1h and 24h, then harvested for microarray analysis (i.e., 8 samples).
In the first of these experiments, cells also were also harvested after 3, 6 and 12 hours of LPS treatment, for a total of 11 samples.
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| Contributor(s) |
Harvey SA, Dangi A, Tandon A, Gandhi CR |
| Citation(s) |
24349206 |
| |
| Submission date |
Aug 19, 2013 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Stephen Alan Kenneth Harvey |
| E-mail(s) |
stephenaharvey@cs.com
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| Organization name |
University of Pittsburgh School of Medicine
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| Department |
Ophthalmology
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| Lab |
EEINS 1014
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| Street address |
203 Lothrop St
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213-2588 |
| Country |
USA |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA215744 |
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