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| Status |
Public on Dec 15, 2013 |
| Title |
Facing warm temperatures during migration – cardiac mRNA responses of two adult sockeye salmon Oncorhynchus nerka populations to warming and swimming challenges |
| Platform organisms |
Oncorhynchus mykiss; Salmo salar |
| Sample organism |
Oncorhynchus nerka |
| Experiment type |
Expression profiling by array
|
| Summary |
The main findings of the current study were that exposing adult sockeye salmon Oncorhynchus nerka to a warm temperature that they regularly encounter during their river migration induced an mRNA-level heat shock response that is exacerbated with swimming. Similar immune defense-related responses were also observed. Microarray analyses revealed that 347 genes were differentially expressed between the cold (12-13° C) and warm (18-19° C) treated fish (P < 0.01), with stress response (GO:0006950; P = 0.014) and response to fungus (GO:0009620; P = 0.003) elevated with warm treatment, while expression for genes involved in oxidative phosphorylation (GO:0006119; P = 0.0019) and electron transport chain (GO:0022900; P = 0.00043) increased in cold-treated fish. By studying single genes with RT-qPCR, warm treatment fish from the Chilko population of O. nerka induced expression of heat shock protein (hsp) 90α, hsp90β and hsp30, as well as interferon-inducible protein (P < 0.05). A Nechako population of O. nerka with a narrower thermal tolerance window than the Chilko population showed even more pronounced responses to the warm treatment. In conclusion, it appears that during their once-in-the-lifetime migration these adult sockeye salmon encounter conditions that induce several cellular defense mechanisms. As river temperatures continue to increase, it remains to be seen whether or not these cellular defenses provide enough protection for all sockeye salmon populations.
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| Overall design |
Two condition experiment; cold treated fish vs. warm treated fish, n=4 in both group
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| Contributor(s) |
Anttila K, Eliason EJ, Kaukinen KH, Miller KM, Farrell AP |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 21, 2013 |
| Last update date |
Dec 16, 2013 |
| Contact name |
Shaorong Li |
| E-mail(s) |
Shaorong.Li@dfo-mpo.gc.ca
|
| Organization name |
Fisheries and Oceans Canada
|
| Department |
Salmon and Freshwater Ecosystems Division
|
| Lab |
Molecular Genetics Lab
|
| Street address |
3190 Hammond Bay Road
|
| City |
Nanaimo |
| State/province |
British Columbia |
| ZIP/Postal code |
V9T 6N7 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL3976 |
GRASP salmonid array, 16k v2.0 chip, updated data files 15 July 2005 |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA215891 |
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