 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 31, 2018 |
| Title |
Gene expression in mouse whole proliferative phase wounds in response to alterations in β-Catenin levels [induced post-injury]. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
β-Catenin is a regulator of cutaneous wound repair. The expression of genes that are modulated in response to changes in β-Catenin levels (stabilization and downregulation) in cutaneous mouse wounds, post injury, during the proliferative phase of repair, was investigated. We are particularly interested in genes that are modulated (in opposite directions) in response to both the induction (stabilization) versus downregulation of β-Catenin. To investigate this, conditional β-Catenin stabilized (Harada, N., et al. EMBO J. 1999 Nov 1;18(21):5931-42) mice were injected with either a Cre-expressing adenovirus, or a GFP-expressing control virus, three days following injury, and wounds were harvested eight days post injury. For conditional knockdown of β-Catenin, mice generated from a cross between conditional β-Catenin knockdown mice (Brault, V. et al., Development. 2001 Apr;128(8):1253-64), and mice that express a tamoxifen-inducible form of Cre (Hayashi, S., and McMahon, A.P., Dev Biol. 2002 Apr 15;244(2):305-18), were treated with Tamoxifen for five days, beginning at the third day after injury. Wounds were harvested eight days after wounding. This provides insight into the identities of genes modulated in response to changes in β-Catenin levels during the proliferative stage of cutaneous wound repair.
|
| |
|
| Overall design |
Total RNA obtained from mouse whole wounds, harvested eight days post injury, in which β-Catenin levels were induced or downregulated (compared to controls) after injury.
|
| |
|
| Contributor(s) |
Alman BA, Bielefeld KA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 23, 2013 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Benjamin Alman |
| Organization name |
Hospital for Sick Children
|
| Department |
Developmental and Stem Cell Biology
|
| Lab |
Alman Lab
|
| Street address |
TMDT, 101 College Street
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
|
| Samples (6)
|
| GSM1214793 |
Adenovirus-GFP_treated_Wildtype_β-Catenin_rep 1 [induced post-injury] |
| GSM1214794 |
Adenovirus-CRE_treated_Stabilized_β-Catenin_rep 1 [induced post-injury] |
| GSM1214795 |
Wildtype_β-Catenin_(Tamoxifen treated)_rep 1 [induced post-injury] |
| GSM1214796 |
Wildtype_β-Catenin_(Tamoxifen treated)_rep 2 [induced post-injury] |
| GSM1214797 |
β-Catenin_Knockdown_(Tamoxifen_Treated)_rep 1 [induced post-injury] |
| GSM1214798 |
β-Catenin_Knockdown_(Tamoxifen Treated)_rep 2 [induced post-injury] |
|
| This SubSeries is part of SuperSeries: |
| GSE50154 |
Gene expression in mouse whole proliferative phase wounds in response to alterations in β-Catenin levels |
|
| Relations |
| BioProject |
PRJNA216915 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE50153_RAW.tar |
15.8 Mb |
(http)(custom) |
TAR |
| GSE50153_non_normalized.txt.gz |
1.9 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...