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| Status |
Public on Nov 21, 2006 |
| Title |
Airway epithelium, large airways, phenotypically normal smokers vs non-smokers, MAS5 (HG-U133 2.0) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Upregulation of Expression of the Ubiquitin Carboxyl Terminal Hydrolase L1 Gene in Human Airway Epithelium of Cigarette Smokers
The microarray data deposited here is from 9 HG-U133 Plus 2.0 GeneChips, from 4 normal non-smokers, and 5 phenotypic normal smokers, all large airways. These data are part of a study aimed at understanding how cigarette smoking modifies neuroendocrine cells, in which microarray analysis with TaqMan confirmation was used to assess airway epithelial samples obtained by fiberoptic bronchoscopy from 81 individuals (normal nonsmokers, normal smokers, smokers with early COPD and smokers with established COPD). Of 11 genes considered to be neuroendocrine cell-specific, only ubiquitin C-terminal hydrolase L1(UCHL1), a member of the ubiquitin proteasome pathway, was consistently upregulated in smokers compared to nonsmokers. Up-regulation of UCHL1 at the protein level was observed with immunohistochemistry of bronchial biopsies of smokers compared to nonsmokers. Interestingly, however, while UCHL1 expression was present only in neuroendocrine cells of the airway epithelium in nonsmokers, UCHL1 expression was also expressed in ciliated epithelial cells in smokers, an intriguing observation in light of recent observations that ciliated cells can are capable of transdifferentiating to other airway epithelium. In the context that UCHL1 is involved in the degradation of unwanted, misfolded or damaged proteins within the cell and is overexpressed in >50% of lung cancers, its overexpression in chronic smokers may represent an early event in the complex transformation from normal epithelium to overt malignancy.
Keywords: smokers vs non-smokers
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| Overall design |
comparison of gene expression in airway epithelial cells of the large airways of healthy smokers vs healthy non-smokers
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| Contributor(s) |
Carolan B, Heguy A, Harvey B, Leopold P, Ferris B, Crystal R |
| Citation(s) |
17108109 |
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| Submission date |
Jun 12, 2006 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Yael Strulovici-Barel |
| E-mail(s) |
yas2003@med.cornell.edu
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| Organization name |
Weill Cornell Medical College
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| Department |
Department of Genetic Medicine
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| Lab |
Crystal
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| Street address |
1300 York Avenue
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (9)
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| GSM114106 |
large airways, non-smoker 004, MAS5 |
| GSM114107 |
large airways, non-smoker 005, MAS5 |
| GSM114108 |
large airways, non-smoker 008, MAS5 |
| GSM114109 |
large airways, non-smoker 030, MAS5 |
| GSM114110 |
large airways, smoker 002, MAS5 |
| GSM114111 |
large airways, smoker 003, MAS5 |
| GSM114112 |
large airways, smoker 023, MAS5 |
| GSM114113 |
large airways, smoker 025, MAS5 |
| GSM114114 |
large airways, smoker 049, MAS5 |
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| This SubSeries is part of SuperSeries: |
| GSE5060 |
Airway epithelium, large and small airways, phenotypically normal smokers, non-smokers, early COPD and COPD |
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| Relations |
| BioProject |
PRJNA104501 |
| Supplementary data files not provided |
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