 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 29, 2014 |
| Title |
A role for H3K4 mono-methylation in facultative heterochromatin assembly and partitioning of chromatin readers |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
Mono-methylation of lysine 4 on histone H3 (H3K4me1) is a well-established feature of enhancers and promoters, although its function is unknown. Remarkably, we find H3K4me1 at the promoter regions of conditionaly-repressed muscle and stimulus-dependent inflammatory response genes in myoblasts. During myogenesis, muscle genes are activated and become H3K4-trimethylated. On the promoters of active genes, we find that H3K4me1 spatially restricts the recruitment of “readers” of H3K4me3, including ING1, which, in turn, recruits Sin3A. Our findings point to a unique role for H3K4 mono-methylation in establishing boundaries that restrict the recruitment of chromatin-modifying enzymes to defined regions within promoters.
|
| |
|
| Overall design |
Mapping of Wdr5, Menin, MLL1, Sin3A, and LSD1 in growing myoblasts (MB) and ING1 in growing myoblasts (MB) and fully differentiated myotubes (MT).
|
| |
|
| Contributor(s) |
Cheng J, Blum R, Dynlacht BD |
| Citation(s) |
24656132 |
| |
| Submission date |
Sep 04, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Roy Blum |
| E-mail(s) |
blumr04@nyumc.org
|
| Phone |
(212) 263-6169
|
| Organization name |
New York University
|
| Department |
Cancer Institute
|
| Lab |
Brian Dynlacht
|
| Street address |
552 First Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (33)
|
|
| Relations |
| BioProject |
PRJNA217995 |
| SRA |
SRP029581 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE50590_ING1_MB_merged_100_15.bed.gz |
70.2 Kb |
(ftp)(http) |
BED |
| GSE50590_ING1_MT_merged_100_15.bed.gz |
82.7 Kb |
(ftp)(http) |
BED |
| GSE50590_MLL1_MB_merged_100_15.bed.gz |
75.9 Kb |
(ftp)(http) |
BED |
| GSE50590_Menin_MB_merged_100_15.bed.gz |
556.9 Kb |
(ftp)(http) |
BED |
| GSE50590_RAW.tar |
156.0 Mb |
(http)(custom) |
TAR (of BED, TDF) |
| GSE50590_Sin3A_MT_merged_100_15.bed.gz |
119.5 Kb |
(ftp)(http) |
BED |
| GSE50590_Wdr5_MB_merged_100_15.bed.gz |
484.6 Kb |
(ftp)(http) |
BED |
SRA Run Selector |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...