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| Status |
Public on Dec 31, 2013 |
| Title |
Transcriptome analysis of Bovine granulosa cells of preovulatory follicles at 30, 60, 90 and 120 days postpartum |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by array
|
| Summary |
Cows in Negative Energy Balance (NEB) may preferentially divert nutrients away from reproduction, thereby experiencing a period of anovulatory anestrus, delayed ovulation of large follicles and a condition of impaired fertility. To better understand the changes occurring in these large follicles as a function of time post-partum granulosa cells of preovulatory follicles have been collected at different times: 30, 60, 90 and 120 days after calving . An analysis of the transcriptome was performed using a global bovine oligo-array microarray to map the differences in genes expression and cellular functions that occur in the follicular microenvironment during the progressive recovery from NEB condition in dairy cow
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| Overall design |
Four time points experiment: 30, 60, 90 and 120 days. Granulosa cells from the 30, 60 and 90 days compare to the 120 days (reference). Biological replicates: 3 from each time point. One replicate per array.
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| Contributor(s) |
Golini VE, Stradaioli G, Sirard M |
| Citation(s) |
24985356 |
| Submission date |
Sep 13, 2013 |
| Last update date |
Jul 07, 2014 |
| Contact name |
Marc-André Sirard |
| E-mail(s) |
Marc-Andre.Sirard@fsaa.ulaval.ca
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| Organization name |
Université Laval
|
| Department |
Sciences Animales
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| Street address |
Offfice 2732, 2440 Hochelaga Blvd.
|
| City |
Québec City |
| State/province |
Quebec |
| ZIP/Postal code |
G1V 0A6 |
| Country |
Canada |
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| Platforms (1) |
| GPL13226 |
Agilent-028298 Embryogene Bovine 45K microarray |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA219169 |
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