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Series GSE51081

Query DataSets for GSE51081

Status

Public on Sep 18, 2014

Title

Opposing Effects of HIF1α and HIF2α on Chromaffin Cell Phenotypic Features and Tumor Cell Proliferation: Insights from MAX [human]

Organism

Homo sapiens

Experiment type

Expression profiling by array

Summary

Pheochromocytomas and paragangliomas (PPGLs) are catecholamine-producing tumors with diverse phenotypic features reflecting mutations in numerous genes, including MYC-associated factor X (MAX). To establish whether PPGL phenotypic differences reflect a MAX-mediated mechanism and opposing influences of HIF2α and HIF1α, we combined observational investigations in PPGLs and gene-manipulation studies in two pheochromocytoma cell lines. In cell lines lacking Max, re-expression of the gene resulted in maturation of phenotypic features and decreased cell cycle progression. In cell lines lacking Hif2α, overexpression of the gene led to immature phenotypic features, failure of dexamethasone to induce differentiation and increased proliferation. HIF1α has opposing actions to HIF2α. These model systems explain the features observed in PPGLs due to mutations of MAX and other PPGL susceptibility genes.

Overall design

88 samples (primary pheochromocytoma (PCC)/paraganglioma tumors) were hybridized onto a cDNA microarray in order to investigate possible heterogeneity within these tumors. This series of tumors is an expansion of GSE19422 that includes nine additional tumors to examine the role of MAX mutations in PCC/PGL.

Contributor(s)

Qin N, de Cubas AA

Citation(s)

24676840

Submission date

Sep 23, 2013

Last update date

Feb 22, 2018

Contact name

Mercedes Robledo

E-mail(s)

mrobledo@cnio.es

Organization name

Spanish National Cancer Research Centre (CNIO)

Department

Human Cancer Genetics Program

Lab

Hereditary Endocrine Cancer Group

Street address

C/ Melchor Fernández Almagro, 3

City

Madrid

State/province

Madrid

ZIP/Postal code

28029

Country

Spain

Platforms (1)

GPL4133

Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)

Samples (9)

More...

GSM1237810	PCC-primary_tumor-rep1 (MAX_1)
GSM1237812	PCC-primary_tumor-rep1 (MAX_4)
GSM1237815	PCC-primary_tumor-rep1 (MAX_5)

This SubSeries is part of SuperSeries:

GSE51087

Opposing Effects of HIF1α and HIF2α on Chromaffin Cell Phenotypic Features and Tumor Cell Proliferation: Insights from MAX

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BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE51081_RAW.tar	62.1 Mb	(http)(custom)	TAR (of TXT)
GSE51081_matrix_all88samples.txt.gz	17.4 Mb	(ftp)(http)	TXT
GSE51081_readme_79re-analyzedSamples.txt	10.2 Kb	(ftp)(http)	TXT
Processed data included within Sample table
Processed data are available on Series record