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Status |
Public on Jun 29, 2006 |
Title |
Xenobiotic response in Drosophila melanogaster: sex dependence of P450 and GST gene induction |
Organism |
Drosophila melanogaster |
Experiment type |
Expression profiling by array
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Summary |
The effect of xenobiotics (phenobarbital and atrazine) on the expression of Drosophila melanogaster CYP genes encoding cytochromes P450, a gene family generally associated with detoxification, was analyzed by DNA microarray hybridization and verified by real time RT-PCR in adults of both sexes. Only a small subset of the 86 CYP genes was significantly induced by the xenobiotics. Eleven CYP genes and three GST genes were significantly induced by phenobarbital, seven CYP and one GST gene were induced by atrazine. Cyp6d5, Cyp6w1, Cyp12d1 and the ecdysone-inducible Cyp6a2 were induced by both chemicals. The constitutive expression of several of the inducible genes (Cyp6a2, Cyp6a8, Cyp6d5, Cyp12d1) was higher in males than in females, and the induced level similar in both sexes. Thus, the level of induction was consistently higher in females than in males. The female-specific and hormonally-regulated yolk protein genes were significantly induced by phenobarbital in males and repressed by atrazine in females. Our results suggest that the numerous CYP genes of Drosophila respond selectively to xenobiotics, providing the fly with an adaptive response to chemically adverse environments. The xenobiotic-inducibility of some CYP genes previously associated with insecticide resistance in laboratory-selected strains (Cyp6a2, Cyp6a8, Cyp12d1) suggests that deregulation of P450 gene expression may be a facile way to achieve resistance. Our study also suggests that xenobiotic-induced changes in P450 levels can affect insect fitness by interfering with hormonally-regulated networks. Keywords: induction by phenobarbital and atrazine in adults drosophila melanogaster of both sex.
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Overall design |
Three independent biological repeat were done for each condition (control, atrazine treatment, phenobarbital treatment). For each microarray experiment, the Cy3 and the Cy5 were swapped between conditions to account for the potential differences in labelling efficiency.
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Contributor(s) |
Le Goff G, Hilliou F, Siegfried BS, Boundy S, Wajnberg E, Sofer L, Audant P, ffrench-Constant RH, Feyereisen R |
Citation(s) |
16876710 |
Submission date |
Jun 22, 2006 |
Last update date |
Mar 16, 2012 |
Contact name |
Gaelle LE GOFF |
E-mail(s) |
legoff@antibes.inra.fr
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Phone |
33 4 92 38 65 78
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Fax |
33 4 92 38 65 55
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Organization name |
INRA
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Lab |
genomique fonctionnelle des insectes
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Street address |
400 route des Chappes
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City |
Sophia-Antipolis |
ZIP/Postal code |
06903 |
Country |
France |
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Platforms (1) |
GPL3874 |
Drosophila melanogaster detoxification array |
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Samples (24)
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GSM116292 |
Female Pheno1 labelled Cy3 vs Female Ctrl1 labelled Cy5 |
GSM116293 |
Female Ctrl2 labelled Cy3 vs Female pheno2 labelled Cy5 |
GSM116294 |
Female Pheno2 labelled Cy3 vs Female Ctrl2 labelled Cy5 |
GSM116295 |
Female Ctrl3 labelled Cy3 vs Female Pheno3 labelles Cy5 |
GSM116296 |
Female Pheno3 labelled Cy3 vs Female Ctrl3 labelled Cy5 |
GSM116297 |
Male Ctrl1 labelled Cy3 vs Male Pheno1 labelled Cy5 |
GSM116298 |
Male Pheno1 labelled Cy3 vs Male Ctrl1 labelled Cy5 |
GSM116299 |
Male Ctrl2 labelled Cy3 vs Male Pheno2 labelled Cy5 |
GSM116300 |
Male Pheno2 labelled Cy3 vs Male Ctrl2 labelled Cy5 |
GSM116301 |
Male Ctrl3 labelled Cy3 vs Male Pheno3 labelled Cy5 |
GSM116302 |
Male Pheno3 labelled Cy3 vs Male Ctrl3 labelled Cy5 |
GSM116303 |
Female Ctrl1 labelled Cy3 vs Female Atz1 labelled Cy5 |
GSM116304 |
Female Atz1 labelled Cy3 vs Female Ctrl1 labelled Cy5 |
GSM116305 |
Female Ctrl2 labelled Cy3 vs Female Atz2 labelled Cy5 |
GSM116306 |
Female Atz2 labelled Cy3 vs Female Ctrl2 labelled Cy5 |
GSM116307 |
Female Ctrl3 labelled Cy3 vs Female Atz3 labelled Cy5 |
GSM116308 |
Female Atz3 labelled Cy3 vs Female Ctrl3 labelled Cy5 |
GSM116309 |
Male Ctrl1 labelled Cy3 vs Male Atz1 labelled Cy5 |
GSM116310 |
Male Atz1 labelled Cy3 vs Male Ctrl1 labelled Cy5 |
GSM116311 |
Male Ctrl2 labelled Cy3 vs Male Atz2 labelled Cy5 |
GSM116312 |
Male Atz2 labelled Cy3 vs Male Ctrl2 labelled Cy5 |
GSM116313 |
Male Ctrl3 labelled Cy3 vs Male Atz3 labelled Cy5 |
GSM116314 |
Male Atz3 labelled Cy3 vs Male Ctrl3 labelled Cy5 |
GSM116748 |
Female Ctrl1 labelled Cy3 vs Female Pheno1 labelled Cy5 |
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Relations |
BioProject |
PRJNA96625 |
Supplementary file |
Size |
Download |
File type/resource |
GSE5125_RAW.tar |
3.7 Mb |
(http)(custom) |
TAR (of GPR) |
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