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Series GSE51317 Query DataSets for GSE51317
Status Public on Oct 01, 2015
Title Sub-chronic transcriptional response in adult male MutaTMMouse following oral exposure to benzo(k)fluoranthene in lung, liver, and forestomach tissues [BkF]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Polycyclic aromatic hydrocarbons (PAHs) are a class of hundreds of structurally similar chemicals ubiquitously present in our environment. They are created during the incomplete combustion of organic materials, such as oil, wood, tobacco, and charbroiled meat. As such, human exposure to mixtures of PAHs can occur through consumption of PAH-containing foods and water, inhalation of polluted air, or dermal contact. Several PAHs have been classified as carcinogenic to humans or probably carcinogenic to humans by the International Agency for Research on Cancer. Benzo(k)fluoranthen is one such compound. In the present study, we sought to determine the dose-dependent changes in gene expression upon oral exposure to benz(a)anthracene in the lung, liver, and forestomach tissues. Adult male MutaTMMouse were exposed to three doses of benzo(k)fluoranthene or vehicle control (olive oil) for 28 days and sacrificed three days after the final exposure.
 
Overall design This experiment examined the pulmonary, hepatic, and forestomach transcriptional response of male mice exposed to benzo(k)fluoranthene for 28 days at three different doses, including D1 (25 mg/kg-bw/day), D2 (50 mg/kg-bw/day), and D3 (100 mg/kg-bw/day) and vehicle control (D0). Each dose group was examined 72 hours following the final exposure. Each dose group had 2-4 biological replicates. There were a total 15, 13, and 14 samples (arrays) from the lung, liver, and forestomach, respectively, included in the final analysis using a two-colour reference design.
 
Contributor(s) Labib S, Williams A, Halappanavar S, Guo CH
Citation(s) 26377693
Submission date Sep 30, 2013
Last update date Feb 02, 2018
Contact name Sarah Labib
Organization name Health Canada
Street address 204b-50 Colombine
City Ottawa
State/province Ontario
ZIP/Postal code K1A0K9
Country Canada
 
Platforms (1)
GPL10787 Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version)
Samples (42)
GSM1242719 D0 Mouse 1_lung [BkF]
GSM1242720 D1 Mouse 7_lung [BkF]
GSM1242721 D2 Mouse 11_lung [BkF]
This SubSeries is part of SuperSeries:
GSE51321 Sub-chronic transcriptional response in adult male MutaTMMouse following oral exposure to 7 individual PAHs in the lung, liver, and forestomach tissues
Relations
BioProject PRJNA222395

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51317_RAW.tar 255.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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