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| Status |
Public on Jul 01, 2016 |
| Title |
mRNA profile of CD8 T cells from chronic HBV patients |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The low frequency of HBV-specific CD8+ T cells in the peripheral blood of CHB patients has limited studies of the mechanisms underlying HBV-induced T cell exhaustion. Similar to the expansion defect displayed in HBV-specific CD8+ T cells, TCR-induced proliferation of global CD8+ T cells is impaired in a fraction of chronic HBV (CHB) patients. Thus, examining the molecular regulation of global CD8+ T cell function in CHB patients may provide insight into the exhaustion of HBV-specific CD8+ T cells.
We used microarrays to detail the global programme of gene expression of CD8 T cells from CHB patients who have not received anti-viral treatment.
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| Overall design |
Fifteen milliliters of blood was drawn from eachof three CHB patients and three healthy donors. PBMCs were enriched using Ficoll, and CD8+ T cells were purified using positive selection beads to a purity of >95% (Miltenyi Biotec, Auburn, CA). Total RNA was extracted using a mirVana isolation kit (Life Technologies, Carlsbad, CA).
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| Contributor(s) |
Wang Y, He Y |
| Citation(s) |
26199080 |
| Submission date |
Oct 21, 2013 |
| Last update date |
Dec 06, 2018 |
| Contact name |
You-Wen He |
| Organization name |
Duke University
|
| Department |
Immunology
|
| Street address |
207 Resarch Drive
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA223293 |
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