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| Status |
Public on Nov 18, 2013 |
| Title |
Transcriptome Sequencing During Mouse Brain Development Identifies Long Non-Coding RNAs Functionally Involved in Neurogenic Commitment |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Transcriptome analysis of somatic stem cells and their progeny is fundamental to identify new factors controlling proliferation versus differentiation during tissue formation. Here we generated a combinatorial, fluorescent reporter mouse line to isolate proliferating neural stem cells, differentiating progenitors and newborn neurons that coexist as intermingled cell populations during brain development. Transcriptome sequencing revealed numerous novel long non-coding (lnc)RNAs and uncharacterized protein-coding transcripts identifying the signature of neurogenic commitment. Importantly, most lncRNAs overlapped neurogenic genes and shared with them a nearly identical expression pattern suggesting that lncRNAs control corticogenesis by tuning the expression of nearby cell fate determinants. We assessed the power of our approach by manipulating lncRNAs and protein-coding transcripts with no function in corticogenesis reported to date. This led to several evident phenotypes in neurogenic commitment and neuronal survival indicating that our study provides a remarkably high number of uncharacterized transcripts with hitherto unsuspected roles in brain development. Finally, we focussed on one lncRNA, Miat, whose manipulation was found to trigger pleiotropic effects on brain development and aberrant splicing of Wnt7b. Hence, our study suggests that lncRNA-mediated alternative splicing of cell fate determinants controls stem cell commitment during neurogenesis.
“LncRNAs control neurogenesis” Aprea, Prenninger, Dori, Monasor, Wessendof, Zocher, Massalini, Ghosh, Alexopoulou, Lesche, Dahl, Groszer, Hiller, Calegari, The EMBO Journal (In Press)
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| Overall design |
mRNA profiles of Proliferating Progenitors, Differentiating Progenitors and Neurons from lateral cortex of E14.5 mouse embryos. Each cell type in three biological replicates.
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| Contributor(s) |
Aprea J, Prenninger S, Dori M, Sebastian Monasor L, Wessendorf E, Zocher S, Massalini S, Ghosh T, Alexopoulou D, Lesche M, Dahl A, Groszer M, Hiller M, Calegari F |
| Citation(s) |
24240175 |
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| Submission date |
Oct 23, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Federico Calegari |
| E-mail(s) |
federico.calegari@tu-dresden.de
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| Organization name |
TU Dresden
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| Department |
Center for Molecular and Cellular Bioengineering (CMCB)
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| Lab |
Calegari Lab
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| Street address |
Fetscherstr. 105
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| City |
Dresden |
| ZIP/Postal code |
01307 |
| Country |
Germany |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA223678 |
| SRA |
SRP031857 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE51606_ALL_GENES.txt.gz |
3.0 Mb |
(ftp)(http) |
TXT |
| GSE51606_RFP+_vs_GFP+.txt.gz |
1.2 Mb |
(ftp)(http) |
TXT |
| GSE51606_RFP-_vs_RFP+.txt.gz |
509.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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