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| Status |
Public on Nov 01, 2013 |
| Title |
Gene expression profiling in neuroblastoma cells upon CHAF1A silencing |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We used an inducible ShRNA system and microarrays to detail the global programme of gene expression underlying neuroblastoma differentiation upon CHAF1A silencing .
CHAF1A is a subunit of the Chromatin Assembly Factor-1 (CAF1) and regulates H3K9-trimethylation. High expression of CHAF1A strongly correlates with neuroblastoma poor prognosis and loss-of-function drives neuronal differentiation in vitro and in vivo.
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| Overall design |
Total RNA was isolated using RNAeasy kit from IMR32 cells transduced with inducible CHAF1A ShRNA. Gene expression profiling was performed in neuroblastoma cells upon CHAF1A silencing over time course (0, 5, and 10 days) in triplicate.
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| Contributor(s) |
Barbieri E, De Preter K, Capasso M, Chen Z, Hsu DM, Tonini GP, Lefever S, Hicks J, Versteeg R, Pession A, Speleman F, Kim ES, Shohet JM |
| Citation(s) |
24335960 |
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| Submission date |
Oct 31, 2013 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Eveline Barbieri |
| E-mail(s) |
exbarbie@txch.org
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| Phone |
832-824-4747
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| Organization name |
Baylor College of Medicine
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| Department |
Department of Pediatrics
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| Lab |
Texas Children's Cancer and Hematology Center
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| Street address |
1102 Bates Street
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| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (9)
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| GSM1256618 |
IMR32 CHAF1A knock down day 5, biological rep1 |
| GSM1256619 |
IMR32 CHAF1A knock down day 5, biological rep2 |
| GSM1256620 |
IMR32 CHAF1A knock down day 5, biological rep3 |
| GSM1256621 |
IMR32 CHAF1A knock down day 10, biological rep1 |
| GSM1256622 |
IMR32 CHAF1A knock down day 10, biological rep2 |
| GSM1256623 |
IMR32 CHAF1A knock down day 10, biological rep3 |
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| Relations |
| BioProject |
PRJNA225961 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE51978_RAW.tar |
43.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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