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| Status |
Public on Nov 08, 2013 |
| Title |
The human cap-binding complex is functionally connected to the nuclear RNA exosome |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
Nuclear processing and quality control of eukaryotic RNA is mediated by the multi-subunit RNA exosome, which utilizes accessory factors to regulate its enzymatic activity. However, the mechanism of exosome recruitment to its ribonucleoprotein (RNP) targets remains poorly understood. Here we disclose a physical link between the human nuclear RNA exosome and the cap-binding complex (CBC). The CBC associates with the ARS2 protein to form CBC-ARS2 (CBCA), and then further connects together with the uncharacterized ZC3H18/NHN1 protein to the nuclear exosome targeting (NEXT) complex, forming CBC-NEXT (CBCN). RNA immunoprecipitation analysis using CBCN factors as baits as well as the combinatorial depletion of CBCN and exosome components underscore the functional relevance of CBC-exosome bridging at the level of target RNA. Specifically, CBCA suppresses read-through transcription of several RNA families by promoting their transcriptional termination. We suggest that the RNP 5’cap links transcription termination to exosomal RNA degradation via CBCN.
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| Overall design |
In total 10 samples; 4 control IP HeLa, 2 Flag- Ars2 IP, 2 Flag-CBP20 IP; and 2 GFP-RBM7 Ips.
The signal intensity data was analyzed with the Affymetrix Tiling Analysis Software (v. 1.1) using parameters: one side upper, 90 bp bandwidth and perfect match only. Output txt files (i.e. result file).
TASParam* files include parameter settings and input file information for the TAS analysis for each result file.
The description of 'ProcessedDatas_TA.xls' file is provided in the 'README.txt'
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| Contributor(s) |
Andersen PR, Domanski M, Kristiansen MS, Stirvall H, Nitni E, Verheggen C, Bunkerborg J, Poser I, Hallais M, Sandberg R, Hyman A, LaCava J, Rout MP, Andersen JS, Bertrand E, Jensen TH |
| Citation(s) |
24270879, 25578728 |
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| Submission date |
Nov 06, 2013 |
| Last update date |
Mar 21, 2017 |
| Contact name |
Edouard BERTRAND |
| Organization name |
CNRS
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| Street address |
1919 Route de Mendes
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| City |
Montpellier |
| ZIP/Postal code |
34293 |
| Country |
France |
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| Platforms (1) |
| GPL4910 |
[Hs35b_P01R] Affymetrix Human Tiling 2.0R Set, Array 1 |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA227077 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE52132_Ars2VsHeLa_signal.txt.gz |
35.4 Mb |
(ftp)(http) |
TXT |
| GSE52132_IPCbpVsIPHela_signal.txt.gz |
36.1 Mb |
(ftp)(http) |
TXT |
| GSE52132_IPIRBM7mHELA_signal.txt.gz |
36.2 Mb |
(ftp)(http) |
TXT |
| GSE52132_ProcessedDatas_TA.xls.gz |
30.8 Kb |
(ftp)(http) |
XLS |
| GSE52132_RAW.tar |
228.0 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE52132_README.txt |
1.4 Kb |
(ftp)(http) |
TXT |
| GSE52132_TASParamARS2VsHeLa.txt.gz |
324 b |
(ftp)(http) |
TXT |
| GSE52132_TASParamIPCbpVsIPHela.txt.gz |
376 b |
(ftp)(http) |
TXT |
| GSE52132_TASParamIPIRBM7mHELA.txt.gz |
319 b |
(ftp)(http) |
TXT |
| Processed data are available on Series record |
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