|
| Status |
Public on Nov 15, 2013 |
| Title |
The Microbial Detection Array for Detection of Emerging Viruses in Clinical Samples - A Useful Panmicrobial Diagnostic Tool |
| Platform organism |
synthetic construct |
| Sample organisms |
Cowpox virus; Monkeypox virus; Orf virus; Eastern equine encephalitis virus; dengue virus type 2; West Nile virus; Yellow fever virus; Rift Valley fever virus; Toscana virus; Dengue virus; sandfly fever Sicilian virus; synthetic construct; Chikungunya virus; Human poliovirus sp.; Usutu virus; Marburgvirus; Zaire ebolavirus; Sandfly fever Naples virus; Hepacivirus hominis; Mammarenavirus lassaense; Orthohantavirus dobravaense; Orthohantavirus hantanense; Orthohantavirus puumalaense; Orthohantavirus seoulense; Orthohantavirus sinnombreense; Orthonairovirus haemorrhagiae |
| Experiment type |
Other
|
| Summary |
Emerging viruses are usually endemic to tropical and sub-tropical regions of the world, but increased global travelling, climate changes and changes in lifestyle are believed to contribute to the spread of these viruses into new regions. For many of them, the disease symptoms are similar to each other, as well as to other more common diseases, making them difficult to diagnose. A rapid identification will help to decide about specific treatment and appropriate case management. Real-time PCR is commonly used for specific virus detection in clinical samples. A diagnostic microarray containing probes for all human viruses, could replace hundreds of specific PCR-reactions and identify all viruses by one assay and thereby remove the need for a clear clinical hypothesis. We show that the Microbial Detection Array successfully identifies emerging viruses present in both non-clinical and clinical samples.
|
| |
|
| Overall design |
Fifteen clinical samples and 27 non-clinical samples (cell culture supernantants or purified viral DNA or RNA) were analyzed for presence of emerging viruses using the MDA microarray.
|
| |
|
| Contributor(s) |
Rosenstierne MW, McLoughlin K, Olesen ML, Pappa A, Engler O, Plumet S, Mirazimi A, Weidmann M, Niedrig M, Fomsgaard A, Erlandsson L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 13, 2013 |
| Last update date |
Nov 16, 2013 |
| Contact name |
Kevin McLoughlin |
| E-mail(s) |
mcloughlin2@llnl.gov
|
| Phone |
(925)423-5486
|
| Organization name |
Lawrence Livermore National Laboratory
|
| Department |
Global Security
|
| Lab |
Pathogen Bioinformatics
|
| Street address |
PO Box 808
|
| City |
Livermore |
| State/province |
CA |
| ZIP/Postal code |
94551 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17919 |
Lawrence Livermore Microbial Detection Array version 2e |
|
| Samples (42)
|
|
| Relations |
| BioProject |
PRJNA227497 |
Less...
More...