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Series GSE5244 Query DataSets for GSE5244
Status Public on Apr 15, 2007
Title Human Uterine Leiomyomata microRNA gene expression
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary MicroRNAs (miRNAs) are a class of small, non-coding RNAs. MiRNAs regulate developmental activity and may be aberrantly expressed in human diseases, including cancer. Expression of miRNAs is tissue or organ specific. Human uterine leiomyomas (ULMs) are the most common neoplasms of women, characterized by the ovarian sex hormone dependence, worse morbidity in black women and genetic heterogeneity. The high incidence of the disease may be associated with developmental defects in uteri. In this study, we examined miRNA expression in 55 ULMs with matched myometrium using spotted microarray platform.
Keywords: Disease state analysis
 
Overall design We selected 206 well-characterized human microRNAs to evaluate miRNA expression in 41 normal myometrium and 55 usual type leiomyomas. The tumor samples were derived from 41 hysterectomies for fibroid uteri. To minimize the variations between cases, tissue samples of tumors and matched normal myometrium from each case was labeled with Cy3 (myometrium) and Cy5 (tumor) respectively and hybridized on the same chip. To reduce the possible spot to spot variation miRNAs were printed in triplicates on the arrays. To identify miRNA whose expression was significantly different between normal and tumor samples, we analyzed all detectable miRNA using both t-test and Significance Analysis of Microarray (SAM) software.
We compared miRNA expression by age, tumor size, ethnicity, tumor phase and number of tumors.

Tumor size:
tumor size < 3cm: GSM114934, GSM114944, GSM114945, GSM114950, GSM114956, GSM114957, GSM114958, GSM114959, GSM114961, GSM114962, GSM114964, GSM114973, GSM114974, GSM114976, GSM114978
tumor size 4-9 cm: GSM114936, GSM114942, GSM114948, GSM114949, GSM114951, GSM114965, GSM114966, GSM114967, GSM114968, GSM114970, GSM114971, GSM114980
tumor size > 10 cm: GSM114935, GSM114937, GSM114938, GSM114939, GSM114940, GSM114941, GSM114943, GSM114946, GSM114947, GSM114952, GSM114953, GSM114954, GSM114955, GSM114960, GSM114963, GSM114969, GSM114972, GSM114975, GSM114977, GSM114979, GSM114981, GSM114982, GSM114983, GSM114984

Ethnicity:
ethnicity-black: GSM114936, GSM114937, GSM114938, GSM114940, GSM114941, GSM114946, GSM114948, GSM114950, GSM114951, GSM114953, GSM114958, GSM114959, GSM114962, GSM114963, GSM114964, GSM114965, GSM114966, GSM114968, GSM114982, GSM114983
ethnicity-white: GSM114934, GSM114935, GSM114939, GSM114943, GSM114944, GSM114945, GSM114949, GSM114952, GSM114954, GSM114955, GSM114960, GSM114961, GSM114970, GSM114971, GSM114973, GSM114974, GSM114975, GSM114976, GSM114978, GSM114979, GSM114981, GSM114984
ethnicity-other: GSM114942, GSM114947, GSM114956, GSM114957, GSM114967, GSM114969, GSM114972, GSM114977, GSM114980

Age:
<40: GSM114938, GSM114946, GSM114967, GSM114973, GSM114975, GSM114976, GSM114977, GSM114981, GSM114982
40-50: GSM114934, GSM114935, GSM114936, GSM114937, GSM114939, GSM114940, GSM114941, GSM114945, GSM114948, GSM114949, GSM114950, GSM114951, GSM114956, GSM114957, GSM114958, GSM114959, GSM114960, GSM114963, GSM114964, GSM114965, GSM114966, GSM114969, GSM114971, GSM114972, GSM114974, GSM114980
>50: GSM114942, GSM114943, GSM114944, GSM114947, GSM114952, GSM114953, GSM114954, GSM114955, GSM114961, GSM114962, GSM114968, GSM114970, GSM114978, GSM114979, GSM114983, GSM114984

Tumor phase:
tumor phase-proliferative endometrium: GSM114934, GSM114936, GSM114937, GSM114941, GSM114943, GSM114945, GSM114951, GSM114954, GSM114955, GSM114956, GSM114958, GSM114959, GSM114960, GSM114961, GSM114965, GSM114966, GSM114967, GSM114968, GSM114969, GSM114970, GSM114971, GSM114972, GSM114973, GSM114975, GSM114976, GSM114977, GSM114978, GSM114984
tumor phase-secretory endometrium: GSM114938, GSM114939, GSM114940, GSM114946, GSM114949, GSM114950, GSM114957, GSM114963, GSM114964, GSM114980, GSM114981, GSM114982
tumor phase-inactive endometrium: GSM114935, GSM114942, GSM114944, GSM114947, GSM114948, GSM114952, GSM114953, GSM114962, GSM114974, GSM114979, GSM114983--

Number of tumors:
number of tumors <5: GSM114935, GSM114936, GSM114944, GSM114949, GSM114952, GSM114957, GSM114960, GSM114973, GSM114974, GSM114975, GSM114977, GSM114979
number of tumors 5-20: GSM114939, GSM114940, GSM114941, GSM114942, GSM114943, GSM114945, GSM114946, GSM114947, GSM114948, GSM114950, GSM114951, GSM114954, GSM114956, GSM114959, GSM114961, GSM114963, GSM114964, GSM114965, GSM114967, GSM114969, GSM114970, GSM114972, GSM114976, GSM114978, GSM114981, GSM114983, GSM114984
number of tumors >20: GSM114934, GSM114937, GSM114938, GSM114953, GSM114955, GSM114958, GSM114962, GSM114966, GSM114968, GSM114971, GSM114980, GSM114982
 
Contributor(s) Wang T, Zhang X, Obijuru L, Aris V, Lee P, Mittal K, Soteropoulos P, Wei J
Citation(s) 17243163
Submission date Jul 06, 2006
Last update date Mar 16, 2012
Contact name Tongsheng Wang
E-mail(s) wang@phri.org
Organization name Public Health Research Institute
Department Center For Applied Genomics
Street address 225 Warren Street
City Newark
State/province NJ
ZIP/Postal code 07103
Country USA
 
Platforms (1)
GPL3879 miRNA expression in Human Uterine Leiomyomata
Samples (51)
GSM114934 MicroRNA Gene Expression in Human Uterine Leiomyomata1
GSM114935 MicroRNA Gene Expression in Human Uterine Leiomyomata2
GSM114936 MicroRNA Gene Expression in Human Uterine Leiomyomata3
Relations
BioProject PRJNA96461

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Supplementary data files not provided
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