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Status |
Public on Apr 15, 2014 |
Title |
The nucleosomal barrier to promoter escape by RNA Polymerase II is overcome by the chromatin remodeler Chd1 [HiSeq] |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
We use a metabolic labeling strategy for directly measuring nucleosome turnover to determine the role of chromatin remodeler Chd1 on chromatin dynamics in mouse embryonic fibroblasts expressing with wildtype-Chd1 and dominant negative K510R-Chd1. We find that expression of K5 10R-Chd1 decreases nucleosome turnover at the promoter and increases turnover within the gene body.
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Overall design |
10 Illumina sequencing samples and 4 Nimblegen array samples
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Contributor(s) |
Skene PJ, Hernandez AE, Groudine M, Henikoff S |
Citation(s) |
24737864 |
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Submission date |
Nov 19, 2013 |
Last update date |
May 15, 2019 |
Contact name |
Jorja Henikoff |
E-mail(s) |
jorja@fhcrc.org
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Phone |
206-667-4850
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Organization name |
Fred Hutchinson Cancer Research Center
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Department |
Basic Sciences
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Lab |
Henikoff
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Street address |
1100 Fairview AV N, A1-162
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City |
Seattle |
State/province |
WA |
ZIP/Postal code |
98109-1024 |
Country |
USA |
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Platforms (2) |
GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (10)
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This SubSeries is part of SuperSeries: |
GSE52349 |
The nucleosomal barrier to promoter escape by RNA Polymerase II is overcome by the chromatin remodeler Chd1 |
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Relations |
BioProject |
PRJNA229126 |
SRA |
SRP033123 |