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| Status |
Public on Dec 26, 2013 |
| Title |
Direct sequencing of polyadenylation sites in fission yeast S. pombe |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We sequenced the 3' ends of polyadenylated transcripts among total RNA in fission yeast wildtype and rdp1∆ cells and mapped the 3'-most nucleotide prior to polyadenylation. We find that mRNA-coding open reading frames have a low number of polyadenylation sites with most of the reads, whereas pericentromeric dg and dh repeats have a high number of polyadenylation sites throughout the transcribed region, mapping to both strands.
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| |
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| Overall design |
direct sequencing of polyadenylated 3' ends of RNA (Helicos)
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| |
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| Contributor(s) |
Yu R, Moazed D |
| Citation(s) |
24374313 |
| |
| Submission date |
Nov 19, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Ruby Yu |
| Organization name |
Harvard University
|
| Department |
Cell Biology
|
| Lab |
Danesh Moazed
|
| Street address |
240 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17955 |
Helicos HeliScope (Schizosaccharomyces pombe) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE52535 |
Determinants of heterochromatic siRNA biogenesis and function |
|
| Relations |
| BioProject |
PRJNA229167 |
| SRA |
SRP033133 |
