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| Status |
Public on Dec 20, 2013 |
| Title |
Genome-wide expression profiling in 293T cells upon depletion of H1.2, Cul4A or PAF1 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Increasing evidence suggests that linker histone H1 can influence distinct cellular processes by acting as a gene-specific regulator. However, the mechanistic basis underlying such H1 specificity and whether H1 acts in concert with other chromatin-altering activities remain unclear. To investigate the cooperative role of H1.2, Cul4A and PAF1 on gene regulation, genome-wide gene expression analysis is carried out in 293T cells expressing control shRNA, H1.2 shRNA, Cul4A shRNA or PAF1 shRNA.
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| Overall design |
Total RNA was isolated from 293T cells expressing NCsh, H1.2sh, Cul4Ash, or PAF1sh
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| Contributor(s) |
Kim K, Lee B, Kim J, Choi J, Kim J, Xiong Y, Roeder RG, An W |
| Citation(s) |
24360965 |
| Submission date |
Nov 19, 2013 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Kyunghwan Kim |
| E-mail(s) |
kyunghki@usc.edu
|
| Phone |
3234424477
|
| Organization name |
University of Southern California
|
| Department |
Biochemistry and Molecular Biology
|
| Street address |
1450 Biggy Street NRT6517
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90033 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA229182 |
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