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Series GSE52600 Query DataSets for GSE52600
Status Public on Oct 20, 2014
Title m6A RNA modification controls cell fate transition in mammalian embryonic stem cells
Organism Homo sapiens
Experiment type Other
Summary N6-methyl-adenosine (m6A) is the most abundant modification on messenger RNAs and is linked to human diseases, but its functions in mammalian development are poorly understood. Here we reveal the evolutionary conservation and function of m6A by mapping the m6A methylome in mouse and human embryonic stem cells. Thousands of messenger and long noncoding RNAs show conserved m6A modification, including transcripts encoding core pluripotency transcription factors. m6A is enriched over 3’ untranslated regions at defined sequence motifs, and marks unstable transcripts, including transcripts turned over upon differentiation. Genetic inactivation or depletion of mouse and human Mettl3, one of the m6A methylases, led to m6A erasure on select target genes, prolonged Nanog expression upon differentiation, and impaired ESC’s exit from self-renewal towards differentiation into several lineages in vitro and in vivo. Thus, m6A is a mark of transcriptome flexibility required for stem cells to differentiate to specific lineages.
Overall design Examing m6A modification differences in two different cell types
Contributor(s) Giallourakis CC, Xing Y, Wang J, Molinie B
Citation(s) 25456834
Submission date Nov 21, 2013
Last update date May 15, 2019
Contact name Jinkai Wang
Organization name University of California, Los Angeles
Street address 650 Charles E. Young Drive South, CHS 33-228
City Los Angeles
State/province CA
ZIP/Postal code 90095-7278
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (12)
GSM1272357 H1A T0 input
GSM1272358 H1A T0 eluate
GSM1272359 H1B T0 input
BioProject PRJNA229454
SRA SRP033229

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Supplementary file Size Download File type/resource
GSE52600_RAW.tar 714.6 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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