|
| Status |
Public on Oct 20, 2014 |
| Title |
m6A RNA modification controls cell fate transition in mammalian embryonic stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
N6-methyl-adenosine (m6A) is the most abundant modification on messenger RNAs and is linked to human diseases, but its functions in mammalian development are poorly understood. Here we reveal the evolutionary conservation and function of m6A by mapping the m6A methylome in mouse and human embryonic stem cells. Thousands of messenger and long noncoding RNAs show conserved m6A modification, including transcripts encoding core pluripotency transcription factors. m6A is enriched over 3’ untranslated regions at defined sequence motifs, and marks unstable transcripts, including transcripts turned over upon differentiation. Genetic inactivation or depletion of mouse and human Mettl3, one of the m6A methylases, led to m6A erasure on select target genes, prolonged Nanog expression upon differentiation, and impaired ESC’s exit from self-renewal towards differentiation into several lineages in vitro and in vivo. Thus, m6A is a mark of transcriptome flexibility required for stem cells to differentiate to specific lineages.
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| |
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| Overall design |
Examing m6A modification differences in two different cell types
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| |
|
| Contributor(s) |
Giallourakis CC, Xing Y, Wang J, Molinie B |
| Citation(s) |
25456834 |
| |
| Submission date |
Nov 21, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Jinkai Wang |
| Organization name |
University of California, Los Angeles
|
| Street address |
650 Charles E. Young Drive South, CHS 33-228
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-7278 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA229454 |
| SRA |
SRP033229 |
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