|
| Status |
Public on Nov 26, 2013 |
| Title |
Transgenic yeast (pAG305GPD-AAE13) vs. control yeast (pAG305GPD-ccdB) |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
The yeast expression vectors used to express AAE13 was constructed as follows: AAE13 (At3g16170) was amplified from the vector pENTR-AAE13. The amplified product was cloned into pCR®8⁄GW⁄TOPO® (Invitrogen). The resulting plasmid was combined with the Advanced Gateway destination vectors pAG305GPD-ccdB (Addgene, Boston, MA) in an attL X attR recombination reaction to generate the vectors pAG305GPD-AAE13. The integrating vector pAG305GPD-AAE13 was introduced into WAT11, which was also transformed empty vector pAG305GPD-ccdB as control (B1, B2, B3 and B4). The transgenic and control yeast cells were cultured in SD-drop out medium at 30 °C under shaken conditions, malonate was fed into cells at final concentration 0.2 mM when the value of OD600 reached at 0.1. The cells were harvested at mid-log phase.
|
| |
|
| Overall design |
Two-condition experiment, Transgenic yeast (pAG305GPD-AAE13) vs. control yeast (pAG305GPD-ccdB). Biological replicates: 4 control replicates, 4 transgenic replicates.
|
| |
|
| Contributor(s) |
Yu O, Wang Y |
| Citation(s) |
24682482 |
| Submission date |
Nov 25, 2013 |
| Last update date |
May 18, 2017 |
| Contact name |
Jinsheng Yu |
| E-mail(s) |
jyu@wustl.edu
|
| Organization name |
Washington University School of Medicine
|
| Department |
Genetics
|
| Lab |
GTAC Lab
|
| Street address |
660 S. Euclid Ave.
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16244 |
Agilent-016322 Yeast (V2) Gene Expression 8x15K Microarray (Probe Name version) |
|
| Samples (4)
|
| GSM1274371 |
AAAE13_replicate_A1_ccdB_replicate_B1 |
| GSM1274372 |
ccdB_replicate_B2 vs. AAAE13_replicate_A2 |
| GSM1274373 |
AAAE13_replicate_A3_ccdB_replicate_B3 |
| GSM1274374 |
ccdB_replicate_B4 vs. AAAE13_replicate_A4 |
|
| Relations |
| BioProject |
PRJNA229864 |
Less...
More...