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Series GSE52969 Query DataSets for GSE52969
Status Public on Dec 16, 2013
Title Expression data from Sar1 isoform overexpressing rat hepatoma cell lines
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary The endoplasmic reticulum (ER) is the site of secretory lipoprotein production and de novo cholesterol synthesis, yet little is known about how these activities are coordinated with each other, or with the activity of the COPII machinery, which transports ER cargo to the Golgi. The Sar1B component of this machinery is mutated in Chylomicron Retention Disorder, establishing that this Sar1 isoform secures delivery of dietary lipids into the circulation.
We used microarrays to investigate the effect of overexpression of Sar1 isoforms and a constitutively active mutant form of Sar1B, Sar1B:H79G, on global gene expression in rat hepatoma cell line, McArdle RH7777 and identified a strong down-regulation of cholesterol biosynthetic gene mRNA expression in the Sar1B:H79G-, but not the wild-type Sar1A- or Sar1B-overexpressing cell lines.
 
Overall design RNA was extracted from cell cultures of control McArdle RH7777, and transgenic lines overexpressing human Sar1-isoforms: one for Sar1A, two for Sar1B and two expressing a constitutively active mutant form of Sar1B, Sar1B:H79G, that cannot complete GTP hydrolysis, and hybridized on Affymetrix GeneChip Rat Genome 230 2.0 arrays.
 
Contributor(s) Jones B, Shoulders C
Citation(s) 24338480
Submission date Dec 04, 2013
Last update date Jul 31, 2017
Contact name Carol Shoulders
Organization name QMUL
Department Centre for Endocrinology
Street address Charterhouse Square
City London
ZIP/Postal code EC1M 6BQ
Country United Kingdom
 
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (25)
GSM1279591 control cell line 1 [ATB2006071812Aaa]
GSM1279592 control cell line 1 [ATB2006071813Aaa]
GSM1279593 control cell line 2 [ATB2006071814Aaa]
Relations
BioProject PRJNA230575

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52969_RAW.tar 107.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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