|
| Status |
Public on Dec 10, 2013 |
| Title |
Transcriptomic analysis of carboxylic acid challenge in Escherichia coli: beyond membrane damage |
| Platform organism |
Escherichia coli |
| Sample organism |
Escherichia coli str. K-12 substr. MG1655 |
| Experiment type |
Expression profiling by array
|
| Summary |
Carboxylic acids are an attractive biorenewable chemical. Enormous progress has been made in engineering microbes for production of these compounds though titers remain lower than desired.
Here we used transcriptome analysis of Escherichia coli during exogenous challenge with octanoic acid (C8) at pH 7.0. This analysis suggests that C8 challenge causes intracellular acidification and membrane damage.
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| Overall design |
Escherichia coli MG1655 was grown to midlog (OD550 ~0.8) with or without 10 mM octanoic acid (pH=7.0) and the RNA was harvested and prepared for Affymetrix Microarray analysis.
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| Contributor(s) |
Royce LA, Boggess E, Fu Y, Dickerson J, Jarboe LR |
| Citation(s) |
24586888 |
| |
| Submission date |
Dec 09, 2013 |
| Last update date |
Mar 08, 2019 |
| Contact name |
Laura Jarboe |
| Organization name |
Iowa State university
|
| Department |
Chemical & Biological Engineering
|
| Street address |
3051 Sweeney Hall
|
| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011 |
| Country |
USA |
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| Platforms (1) |
| GPL3154 |
[E_coli_2] Affymetrix E. coli Genome 2.0 Array |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA230973 |
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