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| Status |
Public on Oct 01, 2015 |
| Title |
CD8+ T cells during acute viral respiratory infection are uniquely differentiated and regulated by multiple inhibitory receptors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Acute viral infection typically generates functional effector CD8+ T cells that aid in pathogen clearance. However, during acute viral lower respiratory infection (LRI), lung CD8+ T cells are functionally impaired and do not optimally control viral replication, while spleen CD8+ T cells specific for the same viral epitopes remain fully functional. To better understand the mechanisms governing lung CD8+ T cell impairment, we used flow cytometry to sort anti-viral CD8+ T cells during viral LRI. Lung and spleen cells were stained with MHC-class I tetramers representing the immunodominant anti-viral CD8+ T cell epitope. We then sorted to high purity: naïve CD8+ T cells, spleen epitope-specific CD8+ T cells, lung epitope-specific CD8+ cells and secondary infection lung epitope-specific CD8+ T cells. We then performed a genome wide transcriptional analysis of these cells to characterize the gene expression profile of lung CD8+ T cell impairment.
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| Overall design |
Epitope-specific lung and spleen CD8+ T cells were obtained using flow cytometric sorting of MHC-class I tetramer stained CD8+ T cells at day 7 after human metapneumovirus (HMPV) infection. Tetramers were specific for the immunodominant M195 epitope (derived from the M protein of HMPV) in B6-Kb0Db0;B7.2 transgenic (B7tg) mice. Naïve CD44-CD62L+ CD8+ T cells were also obtained from the spleen. In a separate group, mice were primed with M195 peptide-pulsed LPS-matured bone marrow-derived dendritic cells, challenged with HMPV two weeks later, and secondary M195-specific CD8+ T cells were obtained at day 7 post-challenge.
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| Contributor(s) |
Erickson JJ, Lu P, Gilchuk P, Joyce S, Shyr Y, Williams JV |
| Citation(s) |
26401005 |
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| Submission date |
Dec 16, 2013 |
| Last update date |
Apr 18, 2017 |
| Contact name |
Pengcheng Lu |
| E-mail(s) |
pengcheng.lu@vanderbilt.edu
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| Organization name |
Vanderbilt University
|
| Street address |
2200 Pierce Avenue
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
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| Platforms (1) |
| GPL11533 |
[MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA231771 |
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