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Status |
Public on Jan 01, 2019 |
Title |
Global expression data from mouse dorsal, ventral and central suprachiasmatic nuclei sub-regions following phase advance inducing light exposure. |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
In mice and other mammals the suprachiasmatic nuclei (SCN) within the brain, synchronises daily rhythms in metabolism, physiology and behaviour to the Earth's local time. Whilst much is known about the SCN's time keeping mechanism, less is known about how it adjusts or resets timing to changes in local time beyond the induction of CRE regulated genes and the differential response of dorsal and ventral sub-regions of the SCN after light exposure known to advance rhythms. As transcription of several clock genes is required for maintaining daily rhythms, we assayed global transcription levels in the dorsal, ventral and central SCN following phase advance inducing light exposure to expand understanding of how the SCN adjusts to new local times.
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Overall design |
Adult C57BL/6 mice were maintained under conditions devoid of timing cues with constant dim red light for at least seven days before receiving a 30m phase advance light pulse (P) or sham (S) treatment. 3.5hrs later mice were sacrificed and brains quickly removed, snap frozen and cryosectioned for subsequent isolation of dorsal, ventral and central SCN by laser catapult microdissection (LCM).
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Contributor(s) |
Brand K, Belle MC, Piggins HD, van der Horst GJ, Jacobs EH |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
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Submission date |
Dec 20, 2013 |
Last update date |
Feb 11, 2019 |
Contact name |
Karl Brand |
E-mail(s) |
brandk@gmail.com
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Organization name |
Erasmus Medical Center
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Department |
Bioinformatics
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Street address |
Dr Molewaterplein 50
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City |
Rotterdam |
ZIP/Postal code |
3015 XG |
Country |
Netherlands |
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Platforms (1) |
GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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Samples (24)
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GSM1296123 |
Pulsed, ventral SCN, replicate 1, batch A |
GSM1296124 |
Pulsed, ventral SCN, replicate 2, batch A |
GSM1296125 |
Pulsed, central SCN, replicate 1, batch A |
GSM1296126 |
Pulsed, central SCN, replicate 2, batch A |
GSM1296127 |
Pulsed, dorsal SCN, replicate 1, batch A |
GSM1296128 |
Pulsed, dorsal SCN, replicate 2, batch A |
GSM1296129 |
Shammed, ventral SCN, replicate 1, batch A |
GSM1296130 |
Shammed, ventral SCN, replicate 2, batch A |
GSM1296131 |
Shammed, central SCN, replicate 1, batch A |
GSM1296132 |
Shammed, central SCN, replicate 2, batch A |
GSM1296133 |
Shammed, dorsal SCN, replicate 1, batch A |
GSM1296134 |
Shammed, dorsal SCN, replicate 2, batch A |
GSM1296135 |
Pulsed, ventral SCN, replicate 3, batch B |
GSM1296136 |
Pulsed, ventral SCN, replicate 4, batch B |
GSM1296137 |
Pulsed, central SCN, replicate 3, batch B |
GSM1296138 |
Pulsed, central SCN, replicate 4, batch B |
GSM1296139 |
Pulsed, dorsal SCN, replicate 3, batch B |
GSM1296140 |
Pulsed, dorsal SCN, replicate 4, batch B |
GSM1296141 |
Shammed, ventral SCN, replicate 3, batch B |
GSM1296142 |
Shammed, ventral SCN, replicate 4, batch B |
GSM1296143 |
Shammed, central SCN, replicate 3, batch B |
GSM1296144 |
Shammed, central SCN, replicate 4, batch B |
GSM1296145 |
Shammed, dorsal SCN, replicate 3, batch B |
GSM1296146 |
Shammed, dorsal SCN, replicate 4, batch B |
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Relations |
BioProject |
PRJNA232367 |
Supplementary file |
Size |
Download |
File type/resource |
GSE53553_RAW.tar |
81.7 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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