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Status |
Public on Nov 01, 2006 |
Title |
Transcriptional response of E. coli to TPEN |
Organism |
Escherichia coli |
Experiment type |
Expression profiling by array
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Summary |
DNA microarrays were used to probe the transcriptional response of E. coli to N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN). Fifty-five transcripts were significantly up-regulated including all of the genes that are regulated by Zur and many genes regulated by Fur. In these same TPEN-treated cells, forty-six transcripts were significantly down-regulated. Keywords: E. coli, TPEN, zinc
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Overall design |
To identify genes that are differentially-expressed in response to TPEN, E. coli BL21(DE3) cells grown in minimal medium until the cultures reached mid-log phase. TPEN was introduced and the cells were cultured for 5 hours; this time was chosen so that the results would correspond to previous transcriptional response studies with excess Zn(II). The cultures containing 5 uM TPEN were analyzed with DNA microarrays (E. coli K12 V2 array slides from MWG-BIOTECH), and the results were compared to those from E. coli cells grown in minimal medium containing no TPEN. A minimum of six slides was used for each experiment (3 slides with one combination of Cy3 and Cy5 dyes and 3 slides with swapped dyes).
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Contributor(s) |
Sigdel TK, Easton JA, Crowder MW |
Citation(s) |
16952965 |
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Submission date |
Jul 19, 2006 |
Last update date |
Mar 16, 2012 |
Contact name |
Tara Sigdel |
E-mail(s) |
tara.sigdel@ucsf.edu
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Phone |
415-502-7919
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Organization name |
University of California San Francisco
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Department |
Surgery
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Lab |
Sarwal Lab
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Street address |
513 parnassus
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City |
San Francisco |
State/province |
CA |
ZIP/Postal code |
94143 |
Country |
USA |
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Platforms (1) |
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Samples (6)
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GSM120166 |
Transcriptional response of E. coli to TPEN (m2) |
GSM120192 |
Transcriptional response of E. coli to TPEN (m1) |
GSM120193 |
Transcriptional response of E. coli to TPEN (m3) |
GSM120194 |
Transcriptional response of E. coli to TPEN (t1) |
GSM120196 |
Transcriptional response of E. coli to TPEN (t2) |
GSM120197 |
Transcriptional response of E. coli to TPEN (t3) |
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Relations |
BioProject |
PRJNA96249 |