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Series GSE5356 Query DataSets for GSE5356
Status Public on Nov 01, 2006
Title Transcriptional response of E. coli to TPEN
Organism Escherichia coli
Experiment type Expression profiling by array
Summary DNA microarrays were used to probe the transcriptional response of E. coli to N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN). Fifty-five transcripts were significantly up-regulated including all of the genes that are regulated by Zur and many genes regulated by Fur. In these same TPEN-treated cells, forty-six transcripts were significantly down-regulated.
Keywords: E. coli, TPEN, zinc
 
Overall design To identify genes that are differentially-expressed in response to TPEN, E. coli BL21(DE3) cells grown in minimal medium until the cultures reached mid-log phase. TPEN was introduced and the cells were cultured for 5 hours; this time was chosen so that the results would correspond to previous transcriptional response studies with excess Zn(II). The cultures containing 5 uM TPEN were analyzed with DNA microarrays (E. coli K12 V2 array slides from MWG-BIOTECH), and the results were compared to those from E. coli cells grown in minimal medium containing no TPEN. A minimum of six slides was used for each experiment (3 slides with one combination of Cy3 and Cy5 dyes and 3 slides with swapped dyes).
 
Contributor(s) Sigdel TK, Easton JA, Crowder MW
Citation(s) 16952965
Submission date Jul 19, 2006
Last update date Mar 16, 2012
Contact name Tara Sigdel
E-mail(s) tara.sigdel@ucsf.edu
Phone 415-502-7919
Organization name University of California San Francisco
Department Surgery
Lab Sarwal Lab
Street address 513 parnassus
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
 
Platforms (1)
GPL3995 MWG E. coli K12 V2 Array
Samples (6)
GSM120166 Transcriptional response of E. coli to TPEN (m2)
GSM120192 Transcriptional response of E. coli to TPEN (m1)
GSM120193 Transcriptional response of E. coli to TPEN (m3)
Relations
BioProject PRJNA96249

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5356_RAW.tar 2.5 Mb (http)(custom) TAR (of GPR)

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