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| Status |
Public on Dec 13, 2017 |
| Title |
Ser7 of RNAPII-CTD facilitates heterochromatin formation by linking ncRNA to RNAi |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by array
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| Summary |
Some long noncoding RNAs (ncRNAs) transcribed by RNA polymerase II (RNAPII) are retained on chromatin, where they regulate RNAi and chromatin structure. The molecular basis of this retention remains unknown. We show that in fission yeast serine 7 (Ser7) of the C-terminal domain (CTD) of RNAPII is required for efficient siRNA generation for RNAi-dependent heterochromatin formation. Surprisingly, Ser7 facilitates chromatin retention of nascent heterochromatic RNAs (hRNAs). Chromatin retention of hRNAs and siRNA generation requires both Ser7 and an RNA-binding activity of the chromodomain of Chp1, a subunit of the RNA-induced transcriptional silencing (RITS) complex. Furthermore, RITS associates with RNAPII in a Ser7- dependent manner. We propose that Ser7 promotes cotranscriptional chromatin retention of hRNA by recruiting the RNA-chromatin connector protein Chp1, which facilitates RNAi-dependent heterochromatin formation. Our findings reveal a function of the CTD code: linking ncRNA transcription to RNAi for heterochromatin formation.
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| Overall design |
Gene expression profile at exponentially-growing phase in the fission yeast mutants of rpb1. Repression of otr1R::ade6+ by heterochromatin in wild-type cells resulted in the formation of red colonies on a solid medium containing a limiting amount of adenine (Low Ade), because lack of the ade6+ gene product results in the accumulation of a red pigments in the cells. By contrast, the ctdS2A and ctdS7A mutants of rpb1 produced a mixture of pink and white colonies showing these mutants are defective in heterochromatin. The microarray analysis was performed respectively on pink and white cells.
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| Contributor(s) |
Kajitani T, Kato H, Chikashige Y, Tsutsumi C, Hiraoka Y, Kimura H, Ohkawa Y, Obuse C, Hermand D, Murakami Y |
| Citation(s) |
29237752 |
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| Submission date |
Dec 20, 2013 |
| Last update date |
Jan 23, 2023 |
| Contact name |
Atsushi Matsuda |
| Organization name |
National Institute of Information and Communications Technology
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| Street address |
588-2, Iwaoka, Iwaoka-cho, Nishi-ku
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| City |
Kobe |
| ZIP/Postal code |
651-2492 |
| Country |
Japan |
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| Platforms (1) |
| GPL15360 |
Agilent-028936 KARC-EX-2010-31 026058 [ProbeName version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA232541 |
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